膀胱癌细胞株T24中侧群细胞的分离和功能鉴定

Isolation and identification of side population cells from human bladder cancer cell line T24

目的观察膀胱癌细胞株T24中是否存在侧群（SP）细胞及其比例，并鉴定其功能。方法利用双波长流氏细胞仪（FACS）检测324中SP细胞的比例，并证实这些SP细胞是否具有癌干细胞的特点。结果T24中SP细胞占34．7％；与非侧群（NSP）细胞比较，SP细胞有更强的生长增殖能力和克隆形成能力（P〈0．05），表达更高的ATP结合转运蛋白G超家族成员2（ABCG2）和干性基因，对放化疗有更强的抵抗能力，有更多的细胞处于G0／G1期（87．4％1：L63．3％，P〈0．05）；分选后的SP和NSP细胞经过约10d的常规培养，SP细胞中NSP的比例占76．2％，而NSP细胞中SP的比例只占2．6％。结论膀胱癌细胞株T24中存在很高比例的SP细胞，而且这些SP细胞有癌干细胞的特点。

Objective To isolate and identify side population （SP） cells from human bladder transitional cell cancer cell line T24. Methods Dual wavelength flourescence-activated cell sorter （FACS） was used to sort T24 cells after Hoechst 33342 labeling, and potential stem cell characteristics of SP cells were analyzed. Results SP cells comprised approximately 34.7% of the total ceils. T24 SP cells had stronger proliferative and colony formation abilities, were also more resistant to chemotherapy and radiotherapy, expressed more ATP-biuding cassette supeffaanily G meanber 2 （ ABCG2 ） and sternness gene, more cells in the G0/Gl phase （87.4% vs 63.3% ,P 〈0.05） and fewer cells in S phase （31. 1% vs 9. 1% , P 〈0.05） than non-SP （NSP） cells. After culture for about 10 days under the normal condition, SP cells still accounted for 23.8%. In contrast, NSP cells only contained 2.6% poorly staining cells. Conclusion SP cells from human bladder transitional cell cancers cell line T24 harbor stem-like cells.