摘要
目的观察肿瘤抑癌基因DBC2(deletioninbreastcancer2)的过表达在乳腺癌MDA—MB-435S细胞中的功能。方法野生型DBC2基因的真核表达载体pEBG—DBC2瞬时转染MDA—MB-435S细胞72h,噻唑蓝(MTF)比色法绘制DBC2转染前后MDA—MB-435S细胞生长曲线;流式细胞术检测DBC2的瞬时过表达对MDA—MB-435S细胞周期的影响,TUNEL方法原位检测DBC2的过表达诱导MDA—MB-435S细胞凋亡。结果DBC2基因在MDA—MB-435S细胞中的过表达可显著抑制该细胞的增殖,同时DBC2基因的过表达可诱导该乳腺癌细胞周期的G,期阻滞(64.05%比71.72%)和细胞凋亡(0.09%比5.29%)。TUNEL实验证实DBC2诱导MDA—MB-435S细胞凋亡的百分比为8%。结论DBC2基因体外抑制乳腺癌细胞生长的功能,可能通过细胞周期G,期停滞,以及诱导细胞凋亡等机制实现。
Objective To gain insight into the biological function of DBC2 in MDA-MB-435S breast cancer cell line in vitro. Methods Transient DBC2 over-expression in MDA-MB-435S cell line was generated by lipofectamine2000 transfection. MTT assay was performed to verify the function of growth inhibition of DBC2 over-expression. The effects of transient DBC2 over-expression was checked by flow cytometry. TUNEL was used to evaluate the apoptosis induced by DBC2. Results The over-expression of DBC2 could significantly inhibit the proliferation of MDA-MB-435S cells and the percentage of inhibition was from 25.95% to 36.43%. Transient over-expression of DBC2 could also induce cell cycle G1 arrest (64.05% vs. 71.72%). Moreover, TUNEL assay showed that the percentage of apoptosis was about 8%. Conclusion Transient over-expression of DBC2 in vitro could inhibit the growth of breast cancer cells, possibly through the mechanisms of inducing G1 cell cycle arrest and apoptosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第3期345-347,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(30400432)
关键词
乳腺癌
肿瘤抑癌基因
增殖
细胞周期
脱噬作用
Breast carcinoma
Tumor-suppressing gene
Proliferation
Cell cycle
Apoptosis