紫外分光光度法测定罗银胶囊中总黄酮含量的不确定度评定

Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method

目的:建立紫外分光光度法测定罗银胶囊中总黄酮含量的测量不确定度评定方法。方法:通过紫外分光光度法测定罗银胶囊中总黄酮含量的测定及方法学验证数据,对来自对照品溶液配制、样品前处理、显色反应、标准曲线拟合及回收率因素所引入的不确定度进行评估,由此计算合成不确定度,最终给出测量结果在95%置信区间下的扩展不确定度。结果:本次实验的罗银胶囊中总黄酮含量测定结果为(31.13±1.61)mg.g-1。结论:建立的不确定度评定法适用于紫外分光光度法测定中药含量的不确定度分析中。

Objective：To evaluate the uncertainty of measurement for determination of total flavonoids in Luoyin soft capsules by UV spectrometery.Method：Through establishing UV spectrometery method determination mode,the factors affecting the uncetainty were deduced,including standard solution preparation,sample pretreat-ment,color reaction,calibration curve and recovery tests.Each component of uncertainty was evaluated,from which the combined uncertainty and finally the extended uncertainty were obtaine in 95% confidence intervals.Result： The determined content of total flavonoids in Luoyin soft capsules of this test is（31.13 ± 1.61） mg.g-1.Conclu-sion：The established mathematic model is applicable to the uncertainty analysis of UV spectrometery method in the determination of traditional Chinese medicine.