疏肝、健脾、疏肝健脾方对肝郁脾虚证模型大鼠外观表征的影响

The action of chaihu shugan san,sijunzi tang and chaishu sijun tang on the apperant GYPX syndrome of Liver-gi stagnation and Spleen-qi deficiency in Rat

目的:比较观察柴胡疏肝散、四君子汤、柴疏四君汤对肝郁脾虚证模型GYPX大鼠表观效应群的影响。方法:大鼠随机分为正常对照组、肝郁脾虚模型组、柴胡疏肝散组、四君子汤组、柴疏四君汤组,共5组,每组10只。后4组大鼠采用慢性束缚+饮食失节+过度疲劳的方法,连续造模4周,正常对照组不予处理。于造模第15 d,中药各组大鼠分别给予柴胡疏肝散4.2 g.kg-1,四君子汤4.53 g.kg-1,柴疏四君汤4.27 g.kg-1ig,1次/d;模型组和对照组分别给予等量蒸馏水,连续14 d。观测实验期间各组大鼠的外观行为、体重及体温的变化。结果:较之于正常对照组,模型大鼠出现外观行为的异常变化和体温与体重的显著降低。较之于模型组,3方给药组大鼠的外观行为均呈现不同程度的改善,其中柴胡疏肝散组大鼠在活动和情绪方面有所改善,四君汤组大鼠在毛发、饮食、大便方面有所改善,柴疏四君汤组大鼠外观行为的诸多方面均有明显改善;3方给药组大鼠的体重和体温均呈不同程度的增加,其中柴疏四君汤组大鼠的体重升高有显著性意义(P<0.05);各给药组大鼠的体温均明显升高(P<0.01),较之于柴胡疏肝散组,柴疏四君汤组大鼠体温升高更明显(P<0.05)。结论:柴疏四君汤对模型大鼠的外观表征的干预效应优于柴胡疏肝散和四君子汤;疏肝方、健脾方、疏肝健脾方与肝郁脾虚证均有不同程度的关联性,疏肝健脾方与肝郁脾虚证的关联性最大。

Objective：To observe and compare the effects of Chaihu Shugan San,Sijunzi Tang and Chaishu Sijun Tang on the apperant syndrome of Liver-gi stagnation and Spleen-qi deficiency（GYPX） in rat.Method： Rats were randomly divided into normal control group,GYPX group,Chaihu Shugan San group,Sijunzi Tang group and Chaishu Sijun Tang group,10 rats in each group.The animals except the controls were experienced by chronic restraint stress ＋ excess fatigue ＋ improper diet to form the model.The model rats were astricted for 3 hours in every morning,swimed for 10 minutes in water（22 ± 1） ℃ and fed every other day,for 28d;normal control group without any stimulation and with natural feeding.On the 15th day,medicated group rats were given Chaishu Sijunzi Tang 4.27 g.kg-1,Chaihu Shugan San 4.2 g.kg-1,Sijunzi Tang 4.53 g.kg-1,1 time /d accordingly;model group and normal group were given distilled water for 14 days.All the changes in behavior,body weight and temperature were observed and recorded.Result： Compared with normal group,the model rats shown the abnormal changes ofbehavior and the decrease of their weight and body temperature.Compared with model group,the behavior of the three medicated groups had improvement to some degrees.Chaihu Shugan San group’s rats increased the level of activity,emotional state of stability;Sijunzi Tang group’s rats improved markedly on the skin-fur appearance,appetite and the state of stool;Chaishu Sijun Tang rats had all respects such as the skin-fur,the level of activity,emotional reaction,sleep or appetite improved clearly.The three treatment groups’body weight and temperature all increased,of which Chaishu Sijun Tang group ’s difference was obvious（P 0.05）.There were significant differences among the three treatment groups in the increased body temperature（P 0.01）.Compared with Chaihu Shugan San group,there was difference of the increased temperature（P 0.05） in Chaishu Sijun Tang group.Conclusion： Chaishu Sijun Tang had better effect on the model rats’apperant GYPX syndrome.There were some relativities among the three prescriptions,of which Chaishu Sijun Tang had the markest relativity with GYPX syndrome.