超滤工艺清开灵注射液解热抗炎作用研究

The effect on antipyretic and Anti-inflammatory of Qingk-ailing Ingection processed by ultrafiltration

目的:探讨超滤工艺对清开灵注射液解热抗炎作用的影响。方法:以解热、抗炎为主要药效学指标,观察不同剂量超滤工艺产品和原工艺产品对大肠杆菌内毒素(LPS)致家兔体温升高的抑制作用,对角叉菜胶致大鼠足跖肿胀急性炎症的影响,以及对大鼠棉球肉芽肿慢性炎症的影响。结果:超滤工艺组和原工艺组在LPS致热1 h后即表现出显著解热作用,与模型组比较有显著性差异,超滤工艺组P<0.01,原工艺组P<0.05,组间无显著性差异;超滤工艺各组和原工艺大剂量组在致炎后2 h有明显抑制大鼠足跖肿胀的作用,与模型组比较,有显著性差异,P<0.05,组间无显著性差异;剂量为14 mL.kg-1超滤工艺组和同等剂量原工艺组棉球湿重与模型组比较,具有显著性差异,P<0.01,P<0.05,棉球干重组间有极显著性差异,P<0.01。结论:超滤工艺制备的清开灵注射液与原工艺产品比较,解热抗炎作用相似,在抑制棉球肉芽肿慢性炎症方面比原工艺产品显示出更强的作用,提示超滤工艺基本可行。

Objective：To study the effect on antipyretic and anti-inflammatory of Qingkailing Ingection（QKL） processed by ultrofiltration（UF）.Method： By using antipyretic and anti-inflammatory as the major pharmacodynamic indicators,to investigate the effect of QKL on inhibition of rabbit increasing temperature induced by LPS,of rat hind paw edema induced by carrageenan and of rat cotton ball granuloma.Result：Both the group of QKL processed by UF and the group of original procedure have obvious effect on antipyretic after 1 hour.Compared with model group,P 0.01,P 0.05;They have obvious effect on anti-inflammatory after 2 hour.Compared with model group,P 0.05,There is no significant difference between the two groups;14 mL.kg-1 dose group of QKL processed by UF and group of original procedure have inhibition of proliferative inflammation.Compared with model group,P 0.01,P 0.05,There is significant difference on dry weight of cotton balls between the two groups,P 0.01.Conclusion：Compared with QKL processed by original procedure,QKL processed by UF has similar action of antipyretic and anti-inflammatory,to some extent,stronger effects.