对比剂稳态磁化率MR灌注成像评价大鼠C6胶质瘤抗血管生成治疗的疗效

Assessment of therapeutic effect of anti-angiogenesis in rat C6 glioma with steady-state susceptibility contrast-enhanced MR perfusion imaging

目的探讨对比剂稳态磁化率MR成像技术评估大鼠C6胶质瘤抗血管生成治疗疗效的价值。方法17只荷瘤大鼠随机分成治疗组(n=8)和对照组(n=9)。治疗组腹腔注射人重组内皮抑素(10 mg/kg),对照组腹腔注射相同剂量的生理盐水,共7天。治疗前、后分别行MR检查,测量肿瘤周边区、肿瘤中央区及对侧相应正常脑实质区的血容量(BV)和血管管径指数(VSIMRI)值,并与病理结果相对照。结果治疗后治疗组与对照组肿瘤体积差异有统计学意义(t=5.26,P<0.05)。治疗后肿瘤中心区及周边区BV和VSIMRI值均有下降,以肿瘤周边区下降更为明显(t=4.14、3.66,P均<0.01)。结论对比剂稳态磁化率MR成像技术评估人重组内皮抑素治疗鼠C6脑胶质瘤具有可行性,能够准确反映治疗前后肿瘤BV和VSI的变化。

Objective To explore the of value steady-state susceptibility contrast-enhanced MRI（SSCE-MRI） in assessment of anti-angiogenesis therapy in rat C6 glioma.Methods Seventeen rats with the bearing-tumor were randomly divided into the therapy group（n =8） and the contrast group（n =9）.Human-recombination endostatin（10 mg/kg） was injected to animals in therapy group,and saline of the same dose was injected to the contrast group for 7 days.Blood volume（BV） and vessel size index（VSI） were measured of tumors in peripheral,central and normal areas with MR pre-and post-therapy.Results After therapy of endostatin,the tumor volume was obviously different between therapy group and contrast group（t =5.26,P 0.05）.BV and VSI MRI of tumor decreased in both peripheral and central areas,particularly in the peripheral area（t =4.14,3.66,all P 0.01）.Conclusion Assessment of therapeutic effect of anti-angiogenesis in C6 glioma is feasible with SSCE-MRI,and it can reflect the changes of BV and VSI MRI pre-and post-therapy.