背向散射积分技术评价大鼠原位肝脏移植病理损害

Evaluation of hepatic pathological damage after orthotopic liver transplantation with integrated backscatter in rats

目的应用背向散射积分(IBS)技术评价原位肝移植(OLT)后大鼠肝脏病理损害。方法32只SD大鼠,40只Wistar大鼠,按不同处理方法分组。建立SD-Wistar OLT模型,分为4组:对照组:8只未予药物干预;CsA组:8只给予环孢素A30 mg/(kg.d);SIN组:8只给予青藤碱40 mg/(kg.d);CsA+SIN组:8只给予青藤碱40 mg/(kg.d)+环孢素15 mg/(kg.d)。正常组:8只Wistar大鼠,为空白对照。术后4天、10天测量肝脏的IBS值[峰-峰强度(PPI),平均强度(AII),强度标准差(SDI)],术后10天处死大鼠取肝脏组织行病理检查。结果术后4天AII对照组和SIN组较正常组、CsA组、SIN+CsA组增高(P<0.05);CsA组、SIN+CsA组高于正常组(P<0.05)。术后10天组间AII对比:CsA组、SIN+CsA组、SIN组较对照组明显下降(P<0.05)。PPI、SDI术后4天、10天各组内及组间比较差异均无统计学意义(P>0.05)。大鼠肝移植后肝脏病理损害程度与IBS呈正相关(r=0.814,P<0.01)。结论测定移植肝脏的IBS有助于判断移植肝脏损害的程度。

Objective To evaluate the hepatic pathological damage after orthotopic liver transplantation（OLT） in rats with integrated backscatter（IBS）.Methods Thirty-two SD rats and 40 Wistar rats were included,and stable OLT models were established except 8 Wistar rats as blank group.The rat models were randomly divided into 4 groups（each n =8）： normal group（given no treatment）,CsA-treated group（30 mg/）,SIN-treated group（40 mg/）,SIN and CsA-treated group（SIN 40 mg/＋CsA 15 mg/）.Hepatic IBS（peak to peak intensity： PPI;average image intensity： AII;standard deviation of image intensity： SDI） was measured on 4th and 10th day after OLT.The rats were sacrificed and a part of liver was cut off for pathological examination.Result Four days later,AII of control and SIN groups were higher than those in other groups（P 0.05）,and of CsA-treated and SIN＋CsA-treated groups were higher than that of blank group（P 0.05）,while no difference of PPI and SDI was detected between each two groups.Ten days later,AII in CsA-treated,SIN＋CsA-treated and SIN-treated groups were lower than that of control group（P 0.05）,no difference of PPI and SDI was detected between each two groups.IBS was positively correlated with liver pathological damage（r =0.814,P 0.01）.Conclusion Detecting hepatic IBS contributes to the diagnosis of the level of liver damage after OLT.