TGF-β1在大鼠胆管损伤修复过程中的表达

TGF-β1 expression during wound healing in rat bile duct

目的:观察TGF-β1在大鼠胆管损伤修复愈合过程中的表达,并探讨其在术后胆道狭窄形成过程中的作用及意义.方法:通过建立大鼠胆管损伤狭窄模型,分别于术后1、2、4和8wk取材行TGF-β1免疫组织化学染色,并观察其阳性细胞数.结果:损伤狭窄胆管组织中TGF-β1主要表达于肉芽组织、成纤维细胞、巨噬细胞、血管内皮细胞.损伤狭窄组TGF-β1的阳性细胞数不同时间均明显高于正常胆管组织(65.47%±5.52%,63.80%±4.32%,59.55%±5.30%,58.17%±6.70%vs12.35%±3.43%,均P<0.01).结论:TGF-β1高表达造成损伤胆管愈合过程中成纤维细胞增殖旺盛、胶原过度合成,从而导致胆管壁纤维化增厚、瘢痕挛缩引发胆管狭窄.

AIM：To investigate the expression of transforming growth factor-β1 （TGF-β1） during wound healing in rat bile duct and analyze its role and significance in the process of biliary stricture formation.METHODS：A rat model of bile duct trauma was established by surgery.The expression of TGF-β1 in traumatic tissue at weeks 1,2,4 and 8 after operation was examined by immunohistochemistry.RESULTS：TGF-β1 was highly expressed in granulation tissue,fibroblasts,macrophages and vascular endothelial cells.The counts of TGFβ1-positive cells in traumatic tissue at different time points were obviously higher than that in normal tissue （65.47% ± 5.52%,63.80% ± 4.32%,59.55% ± 5.30% and 58.17% ± 6.70% vs 12.35% ± 3.43%,respectively;all P 0.01）.CONCLUSION：The high expression of TGF-β1 can induce vigorous proliferation of fibroblasts,oversynthesis of collagen,and fibrosis and thickening of the bile duct wall,thereby resulting in biliary stricture formation.