贵州小型猪幽门螺杆菌的分离及其鉴定

Isolation and Identification of Helicobacter pylori from Guizhou Miniature Pigs

目的对贵州小型猪幽门螺杆菌进行分离鉴定。方法采集1只生长发育异常迟缓的小型猪标本，采用细菌学分离培养、生化鉴定、药敏试验、血清学试验、PCR检测等方法进行鉴定，并对细菌的分子生物学特征进行分析。结果分离到1株细菌，经鉴定为幽门螺杆菌（HPp0812）。用螺杆菌属16SrRNA基因和幽门螺杆菌ureA基因特异引物对HPp0812细菌的PCR扩增为阳性，经核苷酸序列测定分析证实上述序列与幽门螺杆菌参考株基因序列同源性高达99％。结论首次从中国小型猪中分离到了幽门螺杆菌，为进一步研究该菌及开展流行病学调查提供了科学依据。

Objective To isolate and identify Helicobacter pylori in Guizhou miniature pig. Methods Specimens of an abnormal growth and development retarded miniature pig was collected. Bacteria isolation and culture, biochemical identification, susceptibility test, serum agglutination test, PCR assay were conducted. Results One strain of Helicobacter pylori （HPp0812） was isolated and identified. The genome of HPp0812 strain could be amplified by 16S rRNA gene of Helicobacter genus and ure A gene of Helicobacter pylori specific primers in PCR assay. Through determination of the nucleotide sequence, the highest sequence homology of this strain of bacteria could be demonstrated with Helicobacter pylori reference strain in GenB epidemiological ank and it was found to be 99%. Conclusion The strain HPp0812 appears to be the Helicobacterpylori first isolated from miniature pig in China, and it provides reliable scientific data for further research and investigation.