不同时程慢性束缚水浸应激对大鼠胃窦Cajal间质细胞超微结构的影响

Ultrastructural changes in interstitial cells of Cajal in the gastric antrum of rats undergoing water immersion-restraint stress

目的:研究不同时程慢性束缚水浸应激大鼠胃窦Cajal间质细胞(ICC)的超微结构改变.方法:♂SD大鼠48只随机分为6组,即实验3、7、28d组和对照3、7、28d组,每组8只.实验组每日束缚水浸1h,对照组自由摄食饮水;于实验第4、8、29天晨禁食12h后脱颈处死.取胃窦组织2块放入3%戊二醛中固定并电镜下观察ICC超微结构.结果:所有对照组ICC的超微结构均无异常改变,试验3、7、28d各组ICC的超微结构与同期对照组比较均有明显的损害,主要表现为ICC的缝隙连接减少、细胞器减少等,以肌内ICC(ICC-MY)和肌间ICC(ICC-IM)为主;随着应激时间的延长,ICC的超微结构受损逐渐加重.结论:慢性束缚水浸应激可以损伤大鼠胃窦ICC的超微结构.

AIM： To investigate the impact of chronic water immersion-restraint stress on the ultrastructure ofinterstitial cells of Cajal （ICC） in the gastric antrum of rats. METHODS： Forty-eight male Sprague-Dawley rats were randomly and equally divided into six groups： three experimental groups and three matched control groups. The three experimental groups underwent water immersion-restraint stress for one hour daily for 3, 7 and 14 days, re-spectively, while the three control groups were allowed free access to food and water. On days 4, 15 and 28, the rats in both the experimental and control groups were sacrificed. Two pieces of an-trum tissues were taken from each rat and fi xed in 3% glutaraldehyde for electron microscopic examination. RESULTS： ICC were normal in all the controlgroups but showed visible injuries in the three experimental groups. With the prolongation of the stress, the morphological damage be-came more evident in ICC, especially in inter-muscular ICC （ICC-MY） and intramuscular ICC （ICC-IM）. Major ultrastructural changes in ICC included reduced number of gap junctions and organelles. CONCLUSION： Chronic water immersion-re-straint stress can lead to ultrastructural damage in ICC in the gastric antrum of rats.