减肥功能实验动物模型的改良

Modified animal model for the evaluation of weight reduction

目的对传统的评价减肥功能性食品的动物模型进行改良。方法健康雄性SD大鼠50只,随机取20只分为2组,作为传统模型中的对照组和肥胖组,分别喂饲基础饲料和高脂饲料6周,其余30只大鼠喂饲高脂饲料2周后,将体重增加排在后1/3的大鼠作为肥胖抵抗大鼠剔除,其余的按照体重随机分为2组,作为改良模型中的对照组和肥胖组,对照组大鼠转为喂饲基础饲料,肥胖组大鼠继续喂饲高脂饲料4周。实验结束时称体重,体脂含量,计算脂/体比,测量血糖和血脂,并将两种模型进行比较。结果两种模型的肥胖组大鼠的终末体重、体脂含量、脂/体比以及血脂等均显著高于其各自的对照组大鼠,且改良的模型中肥胖大鼠的上述肥胖相关指标都显著高于传统模型中的肥胖大鼠,各项指标组内的差异较小。结论改良的肥胖动物模型优于传统的肥胖动物模型,代表性更好。

Objective To modify the traditional obese model and set up an ideal animal model for evaluating the weight-reducing effect of functional health foods. Method Twenty rats randomly selected from 50 healthy male SD rats and fed with basic diet and high fat diet respectively for 6 weeks were used as control group and obese group for traditional model. Ten rats with the lowest body weight in the rest 30 rats fed with high fat diet for the first 2 weeks were excluded as obesity-resistant rats. The 20 obesity-proneness rats were then randomly divided into control group and obese group respectively for modified model. The rats in the control group of modified model were switched to basic diet and the rats in the obese group of modified model were still fed with high fat diet for another 4 weeks. Then the body weight,fat-pad weight,total fat /body weight,plasma lipids and glucose contents were determined and compared. Results All the obese-related indices in obese groups were significantly worse than those of their respective control rats in both traditional and modified models. The obese-related indices of obese rats in the modified model were worse than those in the traditional model,and the individual variation in a group was relatively smaller in the modified model. Conclusion The modified model is more representative and ideal than the traditional model for evaluating weight-reducing functional food.