不同端粒酶抑制剂对肝癌细胞株蛋白表达的影响

Effects of differentially expressed proteins in hepatocellular carcinoma cell treated by different telomerase inhibitors

目的以表面增强激光解吸及电离飞行时间质谱(SELDI-TOF-MS)技术检测针对人类端粒酶RNA的反义寡核苷酸(hTR-ASODN)、针对hTR的正义寡核苷酸(hTR-SODN)、针对人类端粒酶活性催化亚单位的ASODN(hTERT-ASODN)、针对hTERT的SODN(hTERT-SODN)、没食子酰表没食子儿茶素(EGCG)、3′-叠氮-3′-脱氧胸苷(AZT)、全反式维甲酸(ATRA)和盐酸阿酶素(ADM)8种端粒酶抑制剂分别作用人肝癌细胞SMMC-7721后差异蛋白表达的情况。方法运用SELDI-TOF-MS技术结合配套的弱阳离子交换型芯片(WCX-2)检测8种端粒酶抑制剂分别作用于SMMC-7721细胞后分泌蛋白和胞浆蛋白表达的变化。对照组为未加任何处理因素的SMMC-7721细胞。结果WCX-2蛋白芯片检测发现,8种端粒酶抑制剂作用后细胞株的分泌蛋白和胞浆蛋白分别存在不同程度的表达差异及共同低表达或高表达的差异蛋白。所有差异蛋白分子量均小于10000Da。结论8种端粒酶抑制剂作用后SMMC-7721细胞存在特异性差异蛋白和共性变化的蛋白,这些蛋白可能与端粒酶活性的调控密切相关。

Objective To detect differentially expressed proteins in hepatocellular carcinoma cell line SMMC- 7721 treated separately by eight telomerase inhibitors including antisense oligodeoxynuclectide of human telomerase RNA（ hTR-ASODN）,sense oligodeoxynuclectide of hTR （ hTR-SODN）,ASODN of human telomerase reverse transcriptase （hTERT-ASODN）,SODN of hTERT （ hTERT-SODN）,epigallocatechin gallate （ EGCG）,3′-azido- 3′-deoxythymidine （AZT）,all trans-retinoic acid （ ATRA） and adriamycin （ ADM） using surface enhanced laser desorption /ionization time of flight - mass spectrom （ SELDI-TOF-MS） technology. Methods SELDI-TOF-MS technology and weak cation exchanger （WCX-2） protein chip were used to detect differentially expressed secretory and cytoplasmic proteins of SMMC-7721 cell treated separately by eight telomerase inhibitors. The control group was hepatocellular carcinoma SMMC-7721 cell without any disposal. Results The results of WCX-2 protein chip showed that the secretory and cytoplasmic proteins were differentially expressed in SMMC-7721 cell treated separately by eight telomerase inhibitors. But some proteins were down-regulated or up-regulated together in all experimental groups. The molecular weight of these differential proteins were all less than 10000Da. Conclusion Differentially expressed and common changes of proteins in SMMC-7721 cell treated separately by eight telomerase inhibitors would associate with telomerase activity.