冷凝集素对血型鉴定和交叉配血的影响相关研究分析

Cold Agglutinin on the Identification and Cross Matching Blood Type of Blood Research and Analysis of the Impact and Relevance

目的:探讨婴幼儿血清中高效价冷凝集素对交叉配血试验的影响。方法:ABO血型正反鉴定、吸收放散试验、不规则抗体筛选、交叉配血用直接抗人球蛋白法、聚凝胺法,微柱法配血。结果:采用37℃生理盐水洗涤红细胞,4℃冰箱过夜吸收后,再进行凝聚胺交叉配血或微柱法配血,可有效地排除冷凝集素的干扰,主次侧均无凝集。结论:重视高效价冷凝集素对婴幼儿血型鉴定和交叉配血的影响,高效价冷凝集素所至同型配血不合多为弱凝集,经水浴后凝集消失,证明交叉配血相合,但应加做直接抗人球蛋白试验或凝聚胺配血或微柱法配血,可以排除血清中可能含有其他不规则抗体;亚型同型配血(包括弱抗原与弱抗体配血)不合时也为弱凝集,但37℃水浴后凝集不消失,证明交叉配血不相合,为输血禁忌。

Objective： To investigate the infants in the high titer of serum cold agglutinin on the impact of cross-match test.Method： The identification of ABO blood group positive and negative,absorption elution test,irregular antibody screening,cross matching of blood by direct anti-human globulin method,polybrene method,micro-column method with blood.Result： With 37℃ saline washed red blood cells,4℃ refrigerator overnight absorbed,and then to polybrene cross-matching blood or micro-column method with blood,which can effectively exclude the interference of cold agglutinin,primary and secondary side of no agglutination.Conclusion： Attention to high titer cold agglutinin in infants with blood grouping and cross-matching the impact of high titer cold agglutinin with blood of the same type of sub-Suozhi mostly weak agglutination,agglutination disappeared after by the water bath to prove consistency of cross-matching of blood,it should be added do direct antiglobulin test or polybrene with micro-column method with blood or blood serum can be excluded may contain other irregular antibodies;subtypes with the same type of blood（including the weak antigens and antibodies with a weak blood） when irregularities for weak agglutination,but the 37℃ water bath does not disappear after the condensation to prove that cross-matching of blood mismatched for the blood transfusion taboo.