糖尿病大鼠周围神经bFGF的动态表达及巴曲酶对其的影响

The impact of Batroxobin on the dynamic expression of bFGF in peripheral nerve of diabetic rats

目的探讨碱性成纤维生长因子(bFGF)在糖尿病周围神经病(DPN)大鼠中的动态变化规律;观察巴曲酶对bFGF的影响及其机制,为临床上治疗DPN提供理论依据。方法将大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型,分别于成模后2m和3m时腹腔注射巴曲酶进行干预。通过免疫组织化学和原位杂交双重检测bF-GF在坐骨神经中的表达。结果DM造模后2m时坐骨神经中bFGF的含量明显减少,且随时间变化有统计学差异,巴曲酶治疗后bFGF的表达明显增加。结论DPN大鼠坐骨神经中bFGF表达减少可能参与DPN的发病机制。巴曲酶对DPN有保护作用,其机制可能包括对bFGF的调节。

Objective To investigate the dynamic expression of bFGF in diabetic peripheral neuropathy（DPN）rats and to confirm the impact of Batroxobin on bFGF tentatively and the mechanisms,in order to furnish the theory foundation for the therapy of DPN clinically. Methods Animal models for experimental diabetes were prepared through the peritoneal injection of streptozocin. Batroxobin were injected intraperitoneal respectively in 2 months and 3 months after the formation of DM model for intervention. Through the immunohistochemistry and hybridization in situ,the expression of bFGF in ischiadic nerve of the rats was detected. Results The content of bFGF in ischiadic nerve decreased obviously 2 months after the formation of DM model and it had time-varying statistics diversity. However,the expression of bFGF was markedly increased after the treatment of Batroxobin. Conclusion The decreased expression of bFGF in ischiadic nerve in DPN rats might play an important role in the pathogenesis of DPN. Treatment with Batroxobin is beneficial in the prevention of DPN,possibly through the regulation of the expression level of bFGF in the sciatic nerves of the DPN rats.