流动注射化学发光法测定痕量绿原酸

Determination of Trace Chlorogenic Acid Using Chemiluminescence with Flow Injection

在碱性介质中,绿原酸对luminol-KIO4化学发光反应有强烈抑制作用,且其抑制的程度与绿原酸的浓度的对数呈线性相关.据此,建立了快速、简便、灵敏度高的流动注射—化学发光测定绿原酸的新方法.方法的线性范围为2.0-200.0 ng mL-1,检出限为0.67 ng mL-1(3σ),RSD小于5%(n=5).采用标准加入法,成功的测定了人体血清和金银花茶叶中绿原酸的含量,加标回收率为94.0-105.0%.在流速为2.0 mL.min-1时,完成一次绿原酸的测定只需1分钟.

It was found that chlorogenic acid could obviously inhibit the chemiluminescence generated from lu- minol and luminol-KIO4 by occurring redox in alkaline medium. The decrease of CL intensity was linearly depend- ent on the logarithm of chlorogenic acid concentration in the range from 2 ng·mL^-1to 200 ng·mL^-1 （r2 = 0. 9988） offering a detection limit as low as 0.67 ng·mL^-1 （3σ）. At a flow rate of 2.0 mL · min^-1, one analysis cycle including sampling and washing, could be accomplished in 60 s with a relative standard deviation of less than 5 %. The sensitive flow injection method was applied successfully to determine of chlorogenic acid in pharmaceuti- cal preparations, human serum without any pretreatment procedure, with recovery from 94.0% to 105.0%.