摘要
为探讨硒对氟中毒鸡睾丸组织caspase-3 mRNA表达的影响,将8日龄海兰褐雏鸡180只随机分为3组:Ⅰ组为正常对照组,饲喂全价日粮;Ⅱ组为高氟组,全价日粮中添加氟化钠(NaF),使每千克日粮中氟含量为1 000 mg;Ⅲ组为硒颉颃组,在高氟日粮中添加亚硒酸钠(Na2SeO3),使每千克日粮中硒含量为4 mg。试验期共90 d,分别于试验期第30、60、90天,空腹心脏采血后,剖杀试验鸡,取睾丸组织并检测caspase-3 mRNA表达水平。结果表明:在试验的各个阶段,Ⅲ组中caspase-3 mRNA转录水平均高于同期Ⅱ组。提示硒可以在一定程度上影响氟中毒鸡睾丸组织caspase-3 mRNA的表达水平。
To investigate effects of adding selenium to diets on experssion level of caspase-3 mRNA in teste of fluorositic chicks,180 8-day-age Hy-Line brown chicks were randomly divided into 3 groups,60 per each. Group Ⅰ was the eontrol,group Ⅱ were fed the diets containing 1 000mg/kg of fluorine,and the diet of group Ⅲ contained 1 000mg/kg of sodium fluoride and 4 mg/kg sodium selenite,for 90 days. The experssion level of caspase-3 mRNA in teste was determined at every 30 days. The results showed as follows：During each stage,the experssion level of caspase-3 mRNA of teste in group Ⅲ was higher than that of group Ⅱ （P〈0.05）. It could be concluded that selenium in some degree could effect the expression of caspase-3 mRNA in teste by fluorosis.
出处
《中国家禽》
北大核心
2010年第7期21-23,27,共4页
China Poultry
