高效液相色谱法测定N-乙烯基咔唑的含量

Determination of N-Vinylcarbazole by High Performance Liquid Chromatography

建立了一种操作简单、灵敏度高的N-乙烯基咔唑含量的高效液相色谱测定方法。采用BDS-C18柱,以乙腈∶水=70∶30(体积比)为流动相,流速为1.0mL/min,检测波长为240nm,外标法定量测定N-乙烯基咔唑的含量。N-乙烯基咔唑在0.02～0.2mg/mL范围内呈线性关系,相关系数为0.9999,平均回收率为106.7%,相对标准偏差0.87%。该方法操作简单,灵敏度高,结果准确,适用于N-乙烯基咔唑的含量检测。

A high performance liquid chromatography method was established for the determination of N-vinylcarbazole,which ultilized a BDS C18 column,used acetonitrile-water（70/30,V/V）mixture as mobile phase,and detected at 240 nm with a flow rate of 1.0 mL/min.The linear range of N-vinylcarbazole was 0.02~0.2 mg/mL,the correlation coefficient was 0.9999,and the average recovery was 106.7% with relative standard deviation of 0.87%. The method was simple,quick and accurate,and was suitable for the determination of N-vinylcarbazole.