肺气虚与肺阳虚大鼠肺组织中TNF-α及IL-6免疫表达的实验研究

Experimental study on immunologic expression of TNF-α and IL-6 in lung tissues of rats with insufficiency of the lung-qi and lung-yang

目的观察肺气虚证与肺阳虚证大鼠模型肺组织中肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)的免疫表达变化。方法将健康Wistar大鼠分为对照组、肺气虚证组及肺阳虚证组,各组10只。采用锯末加香烟烟熏法制作肺气虚证模型,将同批烟熏后的大鼠置于寒冷箱内,每日2次,每次2 h,连续7周制作肺阳虚证模型。7周后,取肺组织作病理形态观察并通过免疫组织化学SABC方法观察TNF-α,IL-6的阳性表达。结果与对照组比较,肺气虚证组、肺阳虚证组大鼠肺组织中TNF-α,IL-6总阳性表达面积和平均光密度显著增加(P<0.05或P<0.01);与肺气虚证组比较,肺阳虚证组TNF-α总阳性表达面积的增加更为显著(P<0.05)。结论TNF-α及IL-6参与了肺气虚证、肺阳虚证大鼠模型肺组织的病理发展过程,并可能促进肺气虚证向肺阳虚证的演变。

Objective To observe the change of immunologic expression of tumor necrosis factor-alpha （TNF-α） and interleukin-6 （IL-6） in lung tissues of model rats with insufficiency of the lung-qi and lung- yang. Methods Health wistar rats were divided into control group, insufficiency syndrome of the lung-qi and lung-yang group. The models of insufficiency of the lung-qi were induced by the smoke of saw power and cigarettes for consecutive 7 weeks. The model rats of insufficiency of the lung-yang were placed into the fridge for 2 hours per time for 7 weeks after smoking 30 minutes , every day was 2 times. At the end of 7 weeks, the lung tissue was taken for observing pathologic picture and detecting the immunologic expression of TNF-α and IL-6 by SABC technique of immunohistochemistry. Results Compared with control group, the total positive area and average optical density of TNF-α and IL-6 were obviously increased in insufficiency syndrome of the lungqi and lung-yang group （P 〈 0. 05 or P 〈 0.01 ）. Compared with insufficiency syndrome of the lung-qi group, the total positive area of TNF-α was obviously increased （ P 〈 0.05 ）. Conclusion TNF-α and IL-6 were involved in the pathological process of insufficiency of the lung-qi and lung-yang, and may promote the process from insufficiency of the lung-qi to insufficiency of the lung-yang.