一个糖尿病家系中肾虚证的分布特征及其基因表达谱研究

Distribution Characteristics and Gene Expression Profile of Kidney Deficiency Pattern in a Diabetes Mellitus Pedigree

目的:分析肾虚证在一个糖尿病家系中的分布特征及其差异基因表达谱。方法:对一个2型糖尿病(T2DM)家系的12个成员进行辨证分析和理化检测,选择不同代的典型糖尿病肾虚证3例分别与家系中的正常人1例作基因芯片杂交测试;采用SAM基因芯片数据分析软件筛选差异表达基因作进一步的基因功能分析。结果:肾虚证患者占总家系中追踪到成员的66.7%;通过SAM软件筛选到81个显著表达基因,基因功能注释结果提示免疫反应、糖代谢和氨基酸代谢类基因显著表达。结论:肾虚证在此T2DM家系中呈聚集分布;糖尿病肾虚证与免疫反应及代谢功能类基因的异常表达密切相关。

Objectives：To reveal the distribution characteristics and gene expression profile of kidney deficiency pattern in a T2DM pedigree members.Methods：12 members in T2DM pedigree were closed to make routine physical chemical tests and 3 typical T2DM patients with kidney deficiency and 1 healthy member in the pedigree were selected to subjects of cDNA microarray test.The significantly expressed genes were screened through Significance Analysis of Microarray（SAM）for further functionally annotated.Results：patients with kidney deficiency pattern took up 66.7% of the total investigated family members.81 significantly expressed genes were screened.GO and PATH WAY annotation results of these genes showed that immune and metabolism-related genes and pathways significantly expressed.Conclusions：Distribution of kidney deficiency kidney deficiency pattern showed a familial aggregation trend in T2DM pedigree.The key pathogenesis of T2DM with kidney deficiency pattern is possibly related to immune-genetic disorders.