摘要
目的研究不同病变宫颈组织中Eag1表达情况以及缺氧对宫颈癌Hela细胞膜上Eag1钾离子通道表达的影响。方法采用免疫组化、免疫荧光和RT-PCR法,检测不同病变宫颈组织和宫颈癌Hela细胞中Eag1蛋白的表达情况,以及不同缺氧时间和缺氧浓度对Hela细胞中Eag1 mRNA表达的影响。结果免疫组化结果表明,宫颈上皮内瘤变组织中Eag1蛋白的表达明显高于正常组,宫颈癌组织中Eag1的表达明显高于宫颈上皮内瘤变组;Eag1的表达随临床分期的增高而增加;与病理分级无明显相关性。免疫荧光、RT-PCR结果表明,正常培养的宫颈癌Hela细胞中即有Eag1表达;其表达随缺氧程度加深及缺氧时间的延长明显增强。结论Eag1钾离子通道蛋白有可能成为新的早期筛查和判断宫颈癌生物学行为的诊断指标。Eag1在宫颈癌Hela细胞缺氧感受中起重要的作用。
Objective Immunohistochemistry was employed to detect the expression of Eagl protein in different cervix uteri tissues and CACX Hela cells. Methods We determined the effects of different hypoxia time and hypoxic concentration on the expression of Eagl mRNA in Hela cell by using RT-PCR. Results The expression of Eagl protein in cervical intraepithelial neoplasia (CIN) tissue was significantly higher than that in normal control group,and the expression in CA(;X tissue was remarkably higher than that in CIN group. The expression of Eagl ascended as the development of clinical stage of cervical cancer, but almost irrelevant to pathological grade. Results of immunofluorescence and RT-PCR suggested that the expression of Eagl significantly increased along with the aggravation of hypoxic degree and prolong of hypoxia time. Conclusion Eagl K+ channel protein is possible to become diadynamic criteria for new early screening and CACX biological behavioral judgment. Meanwhile,Eagl plays an important role in hypoxic reception of CACX Hela cell.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第8期899-901,I0001,共4页
Chongqing medicine
基金
国家卫生部课题资助项目(WKJ2007-3-001)
关键词
宫颈癌
钾离子通道
Eag1
cervical cancer
potassium channels
Ether d-go-go