摘要
目的观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)对原代卵巢癌细胞的生长抑制和诱导凋亡情况。方法选择12例患者的原代卵巢癌细胞,每例癌细胞分为6组,分别给予TRAIL 0、5、10、20、50、100μg/L,分别作用24、48、72、96 h。(1)噻唑蓝(MTT)比色法检测TRAIL对原代卵巢癌细胞生长抑制情况。(2)免疫荧光细胞化学法检测原代卵巢癌细胞的凋亡。结果TRAIL对原代卵巢癌细胞的抑制呈剂量依赖性,浓度大于20μg/L后抑制下降;相同浓度TRAIL对原代卵巢癌细胞的抑制呈时间依赖性,72 h后抑制下降;TRAIL浓度相同时随作用时间延长凋亡细胞数增多,5μg/L 24、96 h凋亡细胞数分别为(28±1.18)和(55±4.12),凋亡也呈剂量、时间依赖性。结论TRAIL抑制了原代卵巢癌细胞的增殖、生长并诱导了癌细胞的凋亡。
Objective To investigate the regulatory effects of TRAIL in proliferation and apoptosis of primary human ovarian cancer cell in vitro. Methods Primary human ovarian cancer cell came from twelve patients. Every palient's cell was separated to six groups,and treated with TRAII. (5-100 μg/L) for 24- 96 h respectively. (1)Growth inhibition rates of primary human ovari- an cancer cell were measured with MTT. (2)The apoptosis in primary human ovarian cancer cell was measured with immunofluorescence eylochemistry. Results (1)Growth inhibition rates increased in dose dependent when the concentration is 5 μg/L, 10 μg/L and 20 μg/L,and in time-dependent when the concentration is the same at 24h,48 h,72 h. (2)The apoptosis number increased along with the time lasting. It was 28±1. 18 with the concentration of 5 μg/L at 24 h,and 55±4.12 at 96 h. Conclusion (1)TRAIl. inhibits proliferation and growth of primary human ovarian cancer cell. (2)lt induces apoplosis.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第8期927-929,共3页
Chongqing medicine