13/17罗伯逊易位猪CMYA 3基因多态性分析

Polymorphisms of CMYA3 Gene in 13/17 Robertsonian Translocation Pigs

[目的]对13/17罗伯逊易位杂合子猪[2n=36,rob(13;17)]互交后代中的148个个体进行了CMYA3基因的多态性分析。[方法]采用PCR-RFLP方法。[结果]通过PCR扩增所有个体均能获得507bp的目的片段,PCR产物经限制性内切酶Bsh1236I酶切,琼脂糖凝胶电泳结果表明该基因在此群体中具有A和B2个等位基因,基因频率分别为0.699和0.301;具有AA、AB和BB3种基因型,基因型频率分别为0.615、0.169和0.216。因此,该试验猪群中,A为优势等位基因,AA基因型频率最大。[结论]该研究为13/17罗伯逊易位猪的分子育种和标记辅助选择提供理论依据。

[Objective] The aim was to study the polymorphism of CMYA 3 gene in 148 pigs of hybrid offspring of 13/17 robertsonian translocation heterozygous pigs [2n=37,rob （13;17）] intercrossing.[Method] PCR-RFLP method was adopted.[Result] A 507 bp fragment of CMYA3 gene was obtained by PCR amplification,and then amplification product was digested by restriction nuclease Bsh1236Ⅰ and detected by agarose gel electrophoresis.As a result,both alleles （A and B）of the loci were found in the population.The frequencies of allele A and B were 0.699 and 0.301.The genotype frequencies of AA,AB and BB were 0.615,0.169 and 0.216 respectively.The frequencies of allele A and genotype AA were significantly higher than allele B and genotype BB in populations.[Result] The study will provide theoretical basis for molecular breeding and marker-assisted selection of 13/17 robertsonian translocation pigs.