人甲状腺乳头状癌与正常腺体组织蛋白质组差异表达分析

Analysis of different proteins expression between papillary thyroid carcinoma and normal thyroid tissue

目的比较人甲状腺乳头状癌（papin^thyroidcarcinoma，PTC）与正常腺体组织差异表达的蛋白质，筛选PTC特异的肿瘤标志物并初步探讨其发病机制。方法利用固相pH梯度双向凝胶电泳技术，分离PTC以及正常腺体组织的总蛋白，建立PTC与正常腺体组织的蛋白表达谱；用ImageMaster2DElite5．0图像分析软件，比较两种组织间蛋白表达差异，利用基质辅助激光解吸电离飞行时间质谱对差异蛋白进行鉴定和Swiss．port数据库搜索。结果建立了人PTC与正常腺体组织的蛋白表达谱，通过图像分析发现60个差异蛋白点，经质谱鉴定出17种PTC表达失调蛋白，其中G1／S特异性周期蛋白-D2、锰-超氧化物歧化酶、半乳糖凝集素3等11种蛋白在PTC中过表达，过氧化还原酶_2、热休克蛋白5、热休克蛋白27等6种蛋白在PTC中低表达。结论PTC与正常腺体组织间存在多种差异表达的蛋白质，其可能通过影响细胞周期调控、细胞代谢及参与肿瘤的侵袭与转移等机制参与PTC的发生和发展。

Objective In order to understand the mechanisms of tumor development and identify potential new biomarkers of human papillary thyroid carcinoma（ PTC）, the different proteins expression were investigated and analyzed between PTC and normal thyroid tissue. Methods By using immobilized pH gradient （IPG）-based two-dimensional gel electrophoresis （2-DE）, the total proteins were separated and 2-1）E maps were established for PTC and normal thyroid tissue, respectively. The differential protein spots were analyzed with Image Master 2D EliteS. 0 software, then the peptide mass fingerprinting of different expressed proteins were identified by MALDI-TOF-MS/MS. Also, the functions of the differential expressed proteins were identified through Swiss-port database searching. Results The 2-DE maps of PTC and normal thyroid tissue was established. Sixty differential protein spots were found by image software and seventeen proteins were identified with MALDI-TOF-MS. The biological information of these proteins was identified through Swiss-port database. In comparison with normal thyroid tissue, a total of eleven proteins were up- regulated such as Cyclin-D2, Manganese SOD and Galectin-3; while six proteins were down-regulatted including Peroxiredoxin-2, Hsp70 and Hsp27. Among those up-regulated proteins, some of them were related to cell cycle control, cellular metabolism and invasion and metastasis of the tumor. Conclusions Group of differential expressed proteins between PTC and normal thyroid tissue was identified. These proteins may participate in the tumorigenesis and development of PTC through contributing to cell cycle, invasion, metastasis and cellular metabolism.