二甲基甲酰胺亚急性染毒对小鼠心脏和肝脏的影响及其与脂质过氧化水平的关系

Effect of Subacute Exposed to Dimethylformamide on Heart and Liver of Mice and Its Relationship with Lipid Peroxidation Level

目的研究二甲基甲酰胺(N,N-dimethylformamide,DMF)亚急性染毒对清洁级小鼠心脏和肝脏的影响,并探讨其脂质过氧化损伤机制。方法40只小鼠随机分为4组,分别以1.26、0.63、0.32g/kg经口灌胃染毒,对照组给予生理盐水,每日1次,染毒50d。染毒后宰杀,检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶同功酶(CK-MB)、α-羟丁酸脱氢酶(α-HBDH)的活力和肝脏、心脏组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)含量。结果染毒后,各组间血清中ALT、AST水平无明显变化;各组间肝脏组织中SOD、MDA水平亦无明显变化;NO水平仅低剂量组下降,与对照组比较,差异有统计学意义(P<0.05);血清中LDH、α-HBDH水平在各组间差异无统计学意义,高剂量组和低剂量组CK-MB比对照组及中剂量组均明显升高(P<0.01或P<0.05),心脏组织中SOD和NO水平组间差异无统计学意义,MDA水平升高,且中剂量组和低剂量组较对照组,差异有统计学意义(P<0.05或P<0.01)。结论DMF在该剂量下对小鼠肝脏影响不明显;DMF对小鼠的心肌具有一定的损害;DMF能够增加心肌的脂质过氧化水平,心肌损伤与脂质过氧化水平升高可能具有一定的关联。

[Objective]To study the effect of subacute exposed to dimethylformamide （DMF） on heart and liver of ICR mice,explore its injury mechanism of lipid peroxidation.[Methods]40 mice were randomly divided into four groups,the mice in three dose group were poured into stomach through mouth with DMF at dose of 1.26,0.63 and 0.32 g/kg,and the control group was given normal saline,once a day for 50 days. After treatment period,the mice were sacrificed,the activity of alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,lactate dehydrogenase （LDH）,creatine kinase isoenzyme （CK-MB） and alpha-hydroxybutyrate dehydrogenase （α-HBDH） in serum were tested,and the contents of superoxide dismutase （SOD）,malondialdehyde （MDA） and nitric oxide （NO） in tissues of liver and heart were detected. [Results]After 50 days of treatment,the levels of ALT and AST in serum and SOD and MDA in liver tissue didn＇t changed significantly,and the contents of NO in the low dose group was lower than that in the control group significantly （P0.05）. There was no significant different in levels of LDH、α-HBDH in serum among these groups,but the levels of CK-MB in the high dose group and the low dose group were higher than those in the control group and the medium dose group significantly （P0.01,P0.05）. There was no significant different in contents of SOD and NO in heart tissue among these groups,but the contents of MDA in treatment groups increased,and there were significant different in that between the medium dose group,the low dose group and the control group （P0.05 ,P0.01）.[Conclusion]At the dose of this experiment,DMF had no significant effect on mice＇s livers,but it caused damage on mice＇s hearts. DMF can increase the level of myocardial lipid peroxidation,there is relationship between myocardial injury and increase of lipid peroxidation probably.