武汉市啮齿动物中汉坦病毒分子流行病学研究

Molecular epidemiology of hantavirus carried by rodent hosts in Wuhan,Hubei Province

目的研究湖北省武汉市啮齿动物中汉坦病毒(Hantavirus)流行情况及病毒型别。方法采用间接免疫荧光法(IFA)检测鼠肺中HV抗原;用逆转录聚合酶链式反应(RT-PCR)法扩增阳性样品中HV的部分S片段及部分M片段;构建系统发生树进行系统发生分析及分型。结果在武汉市肾综合征出血热(HFRS)疫区共捕获啮齿动物231只,在9份鼠肺样品中检测到HV抗原,其中5只为褐家鼠,2只为黄胸鼠,2只为小家鼠,病毒携带率为3.90%。用汉城型病毒(SEOV)特异性引物从7份HV抗原阳性样品中扩增出部分S片段(620～999nt)及部分M片段(2001～2301nt)并测定序列。对扩增出的部分S片段和M片段核苷酸序列分析表明,7株病毒与Genebank已提交的SEOV有很高的同源性,均为SEOV。但在用部分S片段及部分M片段核苷酸序列所构建的系统发生树上,7株病毒的聚集模式不同。结论武汉市啮齿动物携带S1和S4亚型汉坦病毒,表现出SEOV在同一地区的遗传多样性。

Aim To investigate the epidemiologlcal features of hantavirus and their genotype in rodents in Wuhan, Hubei Province. Methods Rodents were captured in Wuhan,where hemorrhagic fever with renal syndrome（HFRS） were endemic. Hantavirns antigens in the rat lungs were detected by immunolluoreseence assay （IFA）. Partial S segment （620- 999nt） and partial M segment （2001-2301nt） sequences were amplified by RT-PCR,and then sequenced. Neighbor-joining method was used to construct for phylogenetic analysis. Results A total of 231 rodents were trapped in the epidemic areas,and 9 hantavirus antigens were identified from these lung samples（3.90% ）. Partial S and partial M segment sequences were successfully recovered from 7 samples and determined. Phylogenetic analysis of these sequences indicated that all viruses belonged to Seoul virus （SEOV）,regardless of the sources （Rattus norvegieus and Mus musculus） that they were derived. However,the clustering pattern in the partial S-tree was different from that in the partial M-tree. Conclusion The main genotype of hantavirus carried by Rattus norvegieus and Mus museulus in Wuhan district were S1 and S4 subtype of SEOV, ancl the SEOV types are diverse in Wuhan.