旋毛虫肌肉期幼虫分泌排泄物中46～58kD蛋白的纯化

Purification of 46～58kD Proteins in Excretory-Secretory Products of Trichinella Spiralis Larvae from Muscle

证实了以转移于硝酸纤维膜（NC，paper）的标准蛋白为参照标准（Marker）用SDS－PAGE结合电洗脱技术，分离纯化靶蛋白的可行性。用该技术分离井纯化了旋毛虫肌肉期幼虫分泌排泄物（TsL1－ES）中具免疫原性的抗原组份－46～58kD蛋白，获得近2mg具有明显免疫反应性的高纯度的靶蛋白组份。为进一步研究该特异性抗原组份的诊断价值和保护性免疫作用提供了先决条件。

It was evidenced that seperated and purified specific protein by SDS-PAGEand electroelution technique using standard proteins in nitrocellulose (NC) paper as markerwas practiable. The proteins of TsL1-ES between 46～58kD were purified by these techniques. Acquired purified antigens about 2 000μg showed apparent immunogenicity, highspecificity, and high purity. The quantity of purified proteins obtained are sufficient studiesof immunodiagnosis and protective immunity.