桑色素-Al(Ⅲ)复合物测定蛋白质

Determination of Protein by Morin-Al(Ⅲ) Complex

采用荧光光谱技术,分析研究桑色素-Al（Ⅲ）与生物大分子的相互作用。结果表明：蛋白质对桑色素-Al（Ⅲ）体系有明显的荧光增强作用,在pH=7.7的Tris-HCl缓冲介质中,桑色素-Al（Ⅲ）复合物在520 nm处荧光强度最强,且体系荧光强度的增加与蛋白质的质量浓度在相应范围内呈良好的线性关系。蛋白质（BSA,EA）测定的线性范围分别为：5.00×10-8~3.50×10-6g.mL-1和1.00×10-8~3.00×10-6g.mL-1,检出限分别为2.6×10-9g.mL-1和2.3×10-9g.mL-1。

The reaction between morin-Al（Ⅲ） complex and protein is studied by fluorescent method.Protein can greatly enhance the fluorescence intensity of morin-Al（Ⅲ） in Tris-HCl buffer solution.The strongest fluorescence intensity of the complex is at 520 nm.There is a better linear correlation between the enhancing extent of fluorescence intensity and the concentration of bovine serum albumin（BSA）/egg albumin（EA） in the range of 5.00×10-8~3.50×10-6 g·mL-1/1.00×10-8~3.00×10-6 g·mL-1.The detection limit of BSA/EA is found to be 2.6×10-9/2.3×10-9 g·mL-1 under optimum conditions.