一氧化氮、内皮素-1对体外培养大鼠心肌膜微血管内皮细胞增殖影响的对比研究

A Comparative Study of NO and ET-1 on the Effects of Rat Myocardial Microvascular Endothelial Cell Proliferation

对比研究一氧化氮(NO)供体亚硝基乙酰青酶胺(SNAP)和内皮素-1(ET-1)对体外培养大鼠心肌膜微血管内皮细胞(RMMVECs)增殖的影响。应用不同浓度的SNAP及ET-1作用于RMMVECs,通过MTT法检测SNAP及ET-1对RM-MVECs增殖的影响。与对照组细胞相比,对RMMVECs作用12 h后,浓度为125～1500μmol/L的SNAP均可明显抑制细胞的增殖(P<0.05),抑制率在10.74%～18.80%之间,浓度为0.125～1.5μmol/L的ET-1组有显著的促增殖作用(P<0.05或P<0.01),增殖率在20%～42%之间。在体外,SNAP能够在一定程度上抑制RMMVECs的增殖,而ET-1则能够促进RMMVECs的增殖,结果提示,调节二者在体内的平衡可能对疾病的发生及治疗起着一定的作用。

To compare the effects of ET-1 and S-Nitroso-N-acetyl penicillamine（SNAP）,the donor of NO,on the proliferation of rat myocardial microvascular endothelial cells（RMMVECs）.RMMVECs were incubated with different concentrations of SNAP and ET-1 for 12 h.The cell morphology was observed and cell proliferation ability was determined by MTT.Compared with the control group,the inhibition ratio of cell proliferation of various concentrations of SNAP were 10.74% to 18.80%（P〈0.05）,while for the various concentrations of ET-1,they could promote the ratio of cell proliferation significantly（P〈0.05 or P〈0.01）,the promotion ratio were 20% to 42%.NO could inhibit the proliferation of RMMVECs in vitro,while ET-1 could promote it,which implied that the balance of NO and ET-1 played an important role in the development and treatment of diseases in vivo.