猪肺巨噬细胞FcγR Ⅲ受体基因的克隆与序列分析

Cloning and Sequence Analysis of cDNA Encoding Porcine Alveolar Macrophages FcγR Ⅲ

为研究猪肺巨噬细胞FcγRⅢ的生物学功能,本研究应用RT-PCR技术从猪肺巨噬细胞总RNA中克隆出猪FcγRⅢ的cDNA序列,并对其进行了分析。结果表明,克隆到的序列长820 bp,包含有1个771 bp完整开放阅读框(ORF),与Gen-Bank中登录的猪FcγRⅢ序列(AF237453)的核苷酸同源性为99.9%;与人、牛、马、绵羊、猕猴、狗、猫、小鼠氨基酸同源性分别为61.6%、62.9%、55.3%、62.2%、63.0%、59.0%、61.8%和53.2%;蛋白质分子结构预测结果表明,该分子由信号肽(20个氨基酸)、胞外区(185个氨基酸)、跨膜区(23个氨基酸)和胞内区(28个氨基酸)组成,在胞外区存在2个Ig样结构域。猪肺巨噬细胞FcγRⅢ基因的成功克隆,为进一步研究其结构与功能奠定基础。

To study the biological function of porcine alveolar macrophages FcγR Ⅲ,we cloned cDNA of FcγR Ⅲ gene from total RNA of porcine alveolar macrophages（PAM） by reverse tmnscription-polymerase chain reaction（RT-PCR） and analysised its sequence in this study.Sequence analysis showed that the porcine FcγR Ⅲ cDNA cloned was 820 bp in length which contained 771 bp open reading frame（ORF）.The nucleotide sequence of porcine FcγR Ⅲ shared 99.9% homology with the published sequence（AF237453）.The comparison of the deduced amino acids sequence of porcine FcγRⅢ with those of human,cattle,horse,sheep,monkey,dog,cat amd mouse showed that the amino acids homology similarity were 61.6%,62.9%,55.3%,62.2%,63.0%,59.0%,61.8% and 53.2% respectively.The analysis of deduced amino acids sequence indicated that FcγRⅢ was a typical type I transmembrane protein with two Ig-like domains in extracellular region.The clone of porcine alveolar macrophages FcγR Ⅲ lay the foundation for further study of structure and function of porcine alveolar macrophages FcγRⅢ.