蕨麻猪生长激素基因的同源性和多态性分析

Homology and Polymorphism Analysis of Juema Swine Growth Hormone Gene

为了探索蕨麻猪的遗传多样性,对蕨麻猪生长激素基因序列的多态性进行研究,并选择GenBank中14条序列作为比较研究对象;首次成功克隆了7条蕨麻猪生长激素基因,测出5条蕨麻猪生长激素基因DNA序列,其多态性是内含子1有1个转换和1个颠换;内含子2有2个转换。说明蕨麻猪之间的差异可能与内含子的控制有关。蕨麻猪与其他14种猪之间在编码区和非编码区共检测到核苷酸的变异为155个,内含子有119个,外显子有28个,5′端有8个。其中,颠换104个,转换14个,缺失23个,插入14个。出现碱基取代的位点中,大多数是颠换型突变,颠换与转换之比为7.43∶1,存在颠换偏倚现象。对蕨麻猪生长激素基因同源性百分数和趋异数分析,结果发现,蕨麻猪与小型猪相比同源性较近,蕨麻猪与大型猪同源性较远。蕨麻猪等15种猪生长激素基因序列树状图分析,结果表明,蕨麻猪与Vize猪有较远的亲缘关系;蕨麻猪与贵州榕江香猪亲缘关系最近。

For exploring genetic diversity of Juema pig,researched on the polymorphism of growth hormone gene order of Juema pig,and selected sequences in 14 kinds of pig from GenBank.Full sequence of growth hormone gene of 7 Juema pigs had been cloned successfully for the first time,from which 5 Juema pigs were sequenced respectively,whose polymorphism is intron 1 contains 1 commutation and 1 transversion,intron 2 contains 2 transversions,explain that the difference between Juma