绵羊精液平衡过程中降温速率对精子品质的影响

Influences on Quality of Frozen Semen in the Process of Producing Rate of Temperature Drop for Equilibration on Sheep

本试验研究了河南大尾寒羊精液冷冻颗粒制作过程中,100、150、200和250 mL 4种不同体积水浴平衡降温速率对冻精精子品质的影响。结果表明:①4种水浴平衡处理的降温时间在2.2～3.9 h,以100 mL水浴降温速率最大,250 mL水浴降温速率最小,200和250 mL水浴处理降温速率较为接近。②精液冷冻水浴平衡降温速率前30 min,降温速率应控制在0.73～0.74℃/min之间;30～90 min降温速率应控制在0.11～0.12℃/min之间。③水浴平衡时的降温时间3 h较为适宜,250mL水浴平衡降温的处理对精子造成的冷打击最小,制作的冻精解冻后的精子品质最高。④精子活率:250和200 mL处理间差异不显著(P>0.05);2502、00 mL处理与150、100 mL处理间差异显著(P<0.05)。⑤畸形率:250 mL水浴平衡条件下最低,100 mL水浴平衡条件下最高。250与200 mL处理间精子畸形率差异不显著(P>0.05);250与150、100 mL处理间精子畸形率差异显著(P<0.05)。⑥顶体完整率:250和200 mL较高,两处理间差异不显著(P>0.05);250和200 mL处理与150及100 mL处理间精子顶体完整率差异显著(P<0.05)。

Studied influences on quality of frozen semen in the process of producing rate of temperature drop for equilibration on Henan fat-tailed sheep from 100,150,200 and 250 mL water bath.Results indicated： ① the temperature drop time was 2.2 to 3.9 h that four kind water baths treated.The rate of temperature drop with 100 mL water baths was fastest,250 mL water baths was slowest,200 and 250 mL water baths was a similar in changes.② Optimum rate of temperature drop was 0.73 to 0.74 ℃/min and 0.11 to 0.12 ℃/min from 0 to 30 minute or 30 to 90 minute,respectively.③ Optimum time of temperature drop for equilibration was 3 h.The cooling striking was least and the sperm quality was best with 250 mL water baths treated.④ The sperm motility was not difference with 200 and 250 mL water bath treated,but it was significant difference between 250 and 200 mL treated or 150 and 100 mL treated.⑤ The aberration rate was not difference with 200 and 250 mL water bath treated,it was significant difference between 250 and 200 mL or 150 mL treated.⑥ The rate of acrosomal intactness was significant difference between 150 and 100 mL or 250 and 200 mL treated.