香猪NRAMP1基因多态性与仔猪腹泻的研究

NRAMP1 Gene Polymorphism and Pigs Piglet Diarrhea Research

天然抗性巨噬蛋白1(natural resistance associated macrophage protein 1,NRAMP1)基因是与人、鼠的一些病原微生物(如分支杆菌中的沙门氏伤寒菌等)的易感性和抗性有关的重要候选基因。本研究利用PCR-RFLP技术,分析了香猪的NRAMP1基因第6内含子的NdeⅠ酶切位点的多态性;观测记录哺乳仔猪的腹泻情况,结果发现,NRAMP1基因的第6内含子的NdeⅠ酶切位点在各品种猪表现出丰富的多态性,该酶切位点的基因频率和基因型频率在各猪种中分布趋于一致,香猪不同品系基因型与腹泻指数统计分析结果显示,Ⅰ系、Ⅱ系差异不显著,11世代显著高于10世代。

NRAMP1 gene（natural resistance associated macrophage protein） with the people,a number of pathogenic parasites,such as mouse mycobacterium in Salmonella typhi bacteria＇s susceptibility and resistance of important candidate genes.In this study,PCR-RFLP techniques,analysis of pigs of the NRAMP1 gene intron sixth Nde Ⅰ restriction site polymorphism;observational records of breast-feeding piglet diarrhea and found that NRAMP1 base of intron 6 Nde Ⅰ restriction site in all varieties of pig demonstrated his wealth of polymorphism,the restriction sites of the gene frequencies and genotype frequencies in all pigs in the distribution of consistent,pigs of different strains of genotype and diarrhea index statistics analysis revealed that the department Ⅰ,Ⅱ department no significant difference,with 11 generations were significantly higher than 10 generations.