北京地区人杯状病毒病原学研究

Study on the etiology of human caliciviruses in Beijing

目的了解北京地区人杯状病毒病原学特点。方法收集北京市2007年2～3月病毒性腹泻疑似病例153例,使用逆转录聚合酶链反应法(reverset ranscription polymerase chain reaction,RT-PCR)对粪便标本进行人杯状病毒RNA检测,对RT-PCR阳性标本的PCR产物进行克隆测序。结果检测153例粪便标本,共有54例阳性(54/153,35.29%)。随机选择其中30例PCR产物进行克隆测序,结果表明,28株为诺如病毒(28/30,93.33%),其中GⅡ/4型27株(27/30,90.00%),扎如病毒2株(2/30,6.67%)。结论人杯状病毒是引起北京地区冬季病毒性腹泻的主要病原之一,主要流行株为诺如病毒GⅡ/4型。

Objective To investigate the etiological characteristics of human calicivirusea in Beijing. Methods From February to March 2007, 153 cases of acute nonbacterial gastroenteritis were investigated in Beijing. Human caliciviruses were detected by a reverse transcription polymerase chain reaction （RT-PCR）, and then the PCR products were cloned and sequenced. Results A total of 54 positive cases were detected among 153 patients with acute viral gastroenteritis, with the positive rate of 35.29%. 30 PCR products were randomly selected for sequencing. 28 cases were norovirus （28/30,93.33%）, among which 27 isolates belonged to norovirus GⅡ/4 （27/30, 90.00 % ）. Condusions Human caliciviruses is an important cause of virological diarrhea in winter in Beijing, norovirus G Ⅱ/4 is the predominant genotype.