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沉默Ku70后人耐药肺癌细胞凋亡基因组表达变化及临床意义 被引量：4

The altered expression of apoptosis-related genes and its clinical significance by siRNA-Ku70 silencing in drug-resistant lung cancer cell line
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摘要 目的:探讨沉默Ku70(siRNA-Ku70)后人耐药肺腺癌(A549DDP)细胞系凋亡基因组表达变化及临床意义。方法:应用RT-PCR方法比较Ku70mRNA在人肺腺癌A549细胞系及其耐药肺癌A549DDP细胞系的表达水平;应用PCR-Array方法比较沉默Ku70前后A549DDP细胞凋亡基因组表达水平变化。结果:Ku70mRNA在A549DDP细胞系表达水平显著高于A549细胞系;沉默Ku70后A549DDP细胞促凋亡基因组中BAX、TP53、TP73等呈高表达,抗凋亡基因BFAR及具有双向调节功能的凋亡基因肿瘤坏死因子受体家族成员(TNFRSF 1A)呈低表达(P<0.05)。结论:Ku70mRNA在人耐药非小细胞肺癌细胞系呈现高表达;提示Ku70参与非小细胞肺癌耐药的发生;封闭Ku70可改变耐药肺癌细胞的促/抗凋亡基因组的表达呈促细胞凋亡效应,提示Ku70可能作为上游调控点参与调控部分促/抗凋亡基因组的表达,通过促凋亡表达效应而逆转细胞耐药。 Objective：To investigate the effect of Ku70 silencing on the expression of apoptosis-related genes in human NSLC cell line,A549 and drugresistant A549DDP.Methods：Expression of Ku70mRNA in human A549 cells was examined by RT-PCR.Expression of apoptosis-related genes was examined by PCR-Array assay.Results：The Ku70mRNA was higher in A549DDP cells than in A549 cells.The expression of BAX,TP53 and TP73 was significantly increased whereas the expression of TNFRSF1A and BFAR decreased in siRNA-Ku70 A549DDP cells when compared to those of A549DDP cells.Conclusion：The amount of Ku70 mRNA is higher in A549DDP cells,suggesting that Ku70 is important for drug-resistance;Silencing Ku70 might reverse the drug-resistance in A549DDP cells through regulation of expression of apoptotic genes.
作者 李亚荣 张捷 王珂 刘冰
机构地区 吉林大学白求恩第二临床医院
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2010年第4期312-314,319,共4页 Chinese Journal of Immunology
基金 教育部博士学科点专项科研基金资助项目(200801830069)
关键词 肺癌 细胞耐药 凋亡基因 KU70 siRNA-Ku70 Lung neoplasms Drug resistance Apoptotic gene Ku70 siRNA-Ku70
分类号 R730.52 [医药卫生—肿瘤]
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参考文献14

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同被引文献71

  • 1刘智鑫,刘国津.人非小细胞肺癌细胞药物敏感性检测及其与耐药因子p53抗体和GST-π表达的关系[J].吉林大学学报（医学版）,2005,31(5):795-798. 被引量：9
  • 2史鹏,刘晶,赵凤芹,王欢,李卫红,吴岚.非小细胞肺癌多药耐药因子的表达相关性及临床意义[J].吉林大学学报（医学版）,2007,33(1):151-154. 被引量：3
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引证文献4

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中国免疫学杂志
2010年 第4期

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