毛竹根部解磷细菌的筛选及多样性研究

Diversity of the phosphate solubilizing bacteria isolated from the root of Phyllostachys pubescens

本研究对毛竹(Phyllostachys pubescens)根部(包括根际、根面及内生细菌)解磷细菌进行了筛选,并通过16S rDNA序列分析对其多样性进行了初步研究。从179株毛竹根部细菌中筛选出26株解磷细菌,包括14株根际细菌、8株根面细菌、4株内生细菌。其中,芽孢杆菌BK24的解磷活性最高,其解磷活性达到369.06 mg/L。16S rDNA序列分析表明:26株解磷细菌中,13株属于厚壁菌门(Firmicutes,50.00%),5株属于变形菌门β亚群(Betaproteobacteria,19.23%),4株属于变形菌门γ亚群(Gammaproteobacteria,15.38%),4株属于放线菌门(Actinobacteria,15.38%)。芽孢杆菌属(Bacillus,38.46%)和伯克霍尔德氏菌属(Burkholderia,19.23%)为优势菌属。

26 phosphate-solubilizing bacteria were screened from 179 rhizosphere,rhizoplane and root endophytic bacterial strains of moso bamboo（Phyllostachys pubescens） plants.The phosphate-solubilizing bacteria included fourteen rhizobacteria isolates,eight rhizoplane bacteria isolates,and four endophytic bacteria isolates.Bacillus sp.BK24 showed the highest phosphate solubilizing activity（369.06 mg/L）.Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the strains were clustered into four groups： thirteen strains of Firmicutes（50.00%）,five strains of Betaproteobacteria（19.23%）,four strains of Gammaproteobacteria（15.38%） and four strains of Actinobacteria（15.38%）.The dominant phosphate-solubilizing bacteria were Bacillus and Burkholderia,which was 38.46% and 19.23% respectively.