侧孢短芽孢杆菌S62-9产抗菌物质的分离纯化及部分特性的研究

Study on purification and partial properties of antimicrobial substance from Brevibacillus laterosporus S62-9

为开发新型天然抗菌物质,本研究采用硫酸铵盐析、DEAE-cellulose 52离子交换层析、Sephacryl S-100分子筛层析等步骤对侧孢短芽孢杆菌(Brevibacillus laterosporus)S62-9产的抗菌物质进行分离纯化,并对其部分特性进行研究。结果表明:该抗菌物质对热和pH值稳定性高,且抑菌谱广,可抑制多种革兰氏阳性菌、革兰氏阴性菌和真菌,显示出该抗菌物质具有良好的应用前景。

In order to explore new natural antimicrobial substance,we purified a kind of antimicrobial substance from a strain of Brevibacillus laterosporus S62-9 by using ammonium sulphate precipitation,DEAE-52 column and SephacrylTMS-100 column,etc.Furthermore,the partial properties of the purified substance were studied.The antimicrobial substance was shown thermostable,resistant to pH variation,and a broad spectrum of antagonistic activity against various species of Gram-positive bacteria,Gram-negative bacteria and fungi.All these show that it has a good potential for future development and utilization.