α粒子诱发人胎永生化气管成纤维细胞体外恶性转化细胞生物学特征研究

The study of cell biological character in the neoplastic transformation of immortalizational human fetal tracheal fibroblasts induced by alpha particle irradiation.

研究电离辐射在离体时诱发人细胞恶性转化。方法选用α辐射源诱发ＳＶ４０永生化的人胎气管成纤维细胞恶性转化，观察在细胞恶性转化过程中细胞生物学特性的变化。结果经α粒子照射后细胞形态发生转化；细胞相对存活率与剂量呈依赖性，随着照射剂量的增加，存活率呈指数下降，存活曲线呈单击单靶型；早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；软琼脂克隆形成率随着照后传代次数的增加而明显增高；第４０代细胞接种裸鼠长出肿瘤结节，病理诊断为纤维肉瘤。结论α粒子可诱发人胎永生化气管成纤维细胞体外恶性转化。

Objective\ To study the neoplastic transformation of human cells in vitro induced by alpha particle irradiation.Methods\ Neoplastic transformation of SV40 immortalizational human fetal tracheal fitroblast cells was induced by alpha particle irradiation, and changes of cell biological character during the neoplastic transformation were observed. Result\ The cell morphology was changed after alpha particle irradiation,the relative surviving fraction of the cells irradiated by different doses of alpha particles was dose dependent, and the survival fraction exponential decreased with increasing alpha particles dose. The pattern of cell survival was fitted to single bit single target model. There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time. The colony forming efficincy in soft agar increased with the passaging. There were 4 tumors after SC injection of the 40th passage cells in 5 nude nice.Conclusion\ Neoplastic transformation of immortalizational human fetal tracheal fibroblasts can be induced by alpha particles.