克隆有恶性疟原虫MSP1基因片断的pQE质粒在减毒鼠伤寒杆菌X4064株中的表达

EXPRESSION OF MSP1 GENE FRAGMENT OF PLASMODIUM FALCIPARUM IN ATTENUATED SALMONELLA TYPHIMURIUM X4064 STRAIN

为探讨克隆有恶性疟原虫ＭＳＰ１基因片断的重组ｐＱＥＭ１质粒在减毒鼠伤寒杆菌中的表达。使用转化和转导的方法构建减毒鼠伤寒杆菌Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）；通过ＳＤＳ－ＰＡＧＥ电泳检测减毒鼠伤寒杆菌Ｘ４０６４（ｐＱＥＭ１）和减毒鼠伤寒杆菌Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）的表达。实验结果成功地构建了减毒鼠伤寒杆菌Ｘ４０６４（ｐＱＥＭ１），Ｘ４０６４（ｐＲＥＰ４／ｐＱＥＭ１）的诱导型表达量高于Ｘ４０６４（ｐＱＥＭ１）的组成型表达量。

To determine the expression of recombinant pQEM1 plasmid cloned the MSP1 No 1 fragment of P falciparum in attenuated Salmonella typhimurium X4064,transformation and transduction were used to construct attenuated S typhimurium X4064(pREP4/pQEM1) The expression level of S typhimurium X4064(pREP4/pQEM1) and S typhimurium X4064(pQEM1) was examined by the method of SDS PAGE The result showed that S typhimurium X4064(pREP4/pQEM1) was constructed successfully The inducible expression level of X4064(pREP4/pQEM1) was higher than that of S typhimurium X4064(pQEM1) whose expression was constitutive The experiment indicated that nonregulative expression could reduce the expression level of recombinant pQEM1 plasmid in attenuated S typhimurium X4064