银杏叶化学成分的裂解甲基化气相色谱分析

Determination of multi-chemical constitutes for Ginkgo biloba leaves by gas chromatography with pyrolytic methylation

应用纵型微型炉裂解器直接裂解甲基衍生化-气相色谱法同时测定银杏叶中数种化学成分．样品粉末与衍生化试剂TMAH（25％甲醇）同时注入裂解器进样口，在300℃的进样口瞬间生成了包括5种酚酸类化合物、6种脂肪酸和9种烷基酚在内的甲基衍生物．各种成分的RSD为0．5％～5．9％（n=3）．该方法无需任何样品前处理，样品用量少，为银杏叶中多种化学成分同时测定提供一种快速、简便和准确的方法．

Pyrolytic methylation-gas chromatography using a vertical microfurnace pyrolyzer was applied to analysis of multi-chemical constitutes for G. biloba leaves simultaneously and directly. Pyrolytic methylation of G. biloba leaves in the injector at 300 ~C in the presence of tetramethylammonium hydroxide （TMAH）, allowed quantitative analysis of 5 kinds of phenols and carboxylic acid, 6 kinds of fatty acid and 9 kinds of alkylphenol as their corresponding methyl derivatives, with the relative standard deviations （RSDs） less than 5.9% （n=3）. The developed method allows the analysis small samples without laborious pre-treatment and would be a simple, accurate and highly sensitive way to detect multi-constitutions in Ginkgo biloba leaves.