亚甲蓝光化学法血浆病毒灭活前后血浆成分的变化

The Variation of Plasma Components before and after Virus Inactivation of Methylene Blue Photochemistry

目的探讨亚甲蓝光化学法(methylene blue photochemistry,MB-P)病毒灭活前后血浆有效成分及残留白细胞含量的变化,为临床应用病毒灭活血浆进行治疗提供剂量使用依据。方法对152份全血分离制备的血浆进行MB-P病毒灭活,灭活前后留样,制成新鲜冰冻血浆后融化,测定纤维蛋白原、因子、总蛋白及残留白细胞的含量,同时计算有效成分回收率。结果经MB-P病毒灭活后新鲜冰冻血浆中因子和纤维蛋白原含量明显降低,差异有统计学意义;但总蛋白含量无明显变化,因子回收率达到80.8%,纤维蛋白原回收率为74.9%;残留白细胞计数由(3.0±2.1)×106/L下降到(7.4±1.4)×104/L(n=30),差异有统计学意义(P<0.05)。结论MB-P病毒灭活血浆可以提高血浆输注安全性。建议临床应用MB-P病毒灭活血浆应在原使用剂量基础上增加25%的用量,以保证临床治疗效果。

Objective To explore the variation of the effective components of plasma and the amount of residual leukocytes before and after virus inactivation of methylene blue photochemistry,and to provide envidences for the dose of virus inactivated plasma in the clinical usage.Methods The plasma separated from 152 samples of whole blood was inactivated by methylene blue photochemistry.The samples were kept before and after the inactivation and fresh frozen plasma were prepared.Then they were melt to assay the level of fibrinogen,Ⅷ factors,total protein and the residual leukocytes.Meanwhile,to calculate the recovery rate of the effective components.Results The levels of Ⅷ factors and fibrinogen in the fresh frozen plasma were significantly decreased after virus inactivation of methylene blue photochemistry（P〈0.05）.No significant difference in the level of total protein was found（P〉0.05）.The recovery rate of Ⅷ factors reached up to 80.8%.The recovery rate of fibrinogen was 74.9%.The amount of the residual leukocytes decreased to（7.4±1.4）×10^4/L from（3.0±2.1）×10^6/L（n=30） and the difference was significant（P0.05）.Conclusion Inactivated plasma with methylene blue photochemistry can improve the safety of plasma transfusion.We suggested the dose of inactivated plasma with methylene blue photochemistry be increased by 25% to make sure the clinical therapeutic effects.