邯郸地区乙肝病毒感染者血清学模式及基因型检测结果分析

Analysis on Test Results of Serological Patterns and Genotypes Among People with HBV Infection in Handan Area

目的研究邯郸地区乙肝病毒感染者血清学表达模式的流行病学和基因型分布,为完善预防、诊断、治疗病毒感染的策略和方法提供科学依据。方法应用酶联免疫吸附(ELISA)检测乙肝病毒感染者血清HBsAg、HBsAb、HBeAg、HBeAb和HBcAb。型特异性引物PCR检测其基因型。对结果进行统计分析。结果306份标本中HBsAg/HBeAg/HBcAb(M1),男:65份,年龄(31.6±15.1)岁。女:40份,年龄(26.82±9.24)岁。HBsAg/HBeAb/HBcAb(M2),男:52份,年龄(35.6±14.8)岁。女:58份,年龄(37.8±13.39)岁。HBsAg/HBcAb(M3),男:44份,年龄(39.4±14.0)岁。女:24份,年龄(39.4±13.0)岁。M1和M3模式男女比率差异无统计学意义(P>0.05)。M2模式男女相比差异有统计学意义(P<0.05)。统计图表示三种血清学模式表达的高峰期都集中在20～30岁之间,对M1血清学模式105份样本进行基因分型,95份样品成功分型,全部为C基因型。结论邯郸市区乙肝病毒感染者在3种常见血清学表达模式表达上M2男女比率差异有统计学意义,女性高于男性,20～30岁为携带和传播的高峰期,C型为优势基因型。

Objective To study the epidemiology of serological expression pattern and genotype distribution among people infected with HBV in Handan area,and to provide a scientific basis in improving the strategies and methods of prevention,diagnosis,and treatment of HBV infection.Methods Enzyme-linked immunosorbent assay （ELISA） was used to detect HBsAg,HBsAb,HBeAg,HBeAb,and HBcAb in people with HBV infection.The genotypes were detected by PCR using type-specific primers.The results were statistically analyzed.Results In 306 specimens,HBsAg/HBeAg/HBcAb（M1）,male：65,age：（31.6±15.1） years;female：40,age：（26.82±9.24） years.HBsAg/HBeAb/HBcAb（M2）,male：52,age：（35.6±14.8.） years;female：58,age：（37.8±13.39） years.HBsAg/HBcAb（M3）,male：44,age：（39.4±14.0） years;female：24,age：（39.4±13.0） years.No statistically significant difference was found in the distribution rate of M1 and M3 models between men and women （P〉0.05）.There were statistically significant differences in M2 model between men and women （P〈0.05）.In the diagram of age distribution of three common patterns,the peaks were concentrated in the 20～30 years old.105 specimens in M1 model were detected the genotype,and 95 were identified successfully.All of them were the C genotype.Conclusions The distribution difference between men and women is statistically significant in the M2 about hepatitis B virus infection in Handan area.The proportion of women is more than that of the men.The peak period of carrying and spreading HBV is between 20 and 30 years old.C-type is the dominant genotype.