临床分离鲍曼不动杆菌耐药性检测及其主动外排机制的确证

Detection of Drug Resistance of Clinical Separated Acinetobacter baumannii and Confirmation of Its Active Efflux Mechanism

目的对我院分离的鲍曼不动杆菌进行耐药性分析并初步探讨是否存在药物主动外排机制。方法K-B法对临床分离的鲍曼不动杆菌进行药敏试验,CCCP联合不同抗菌药物测定MIC值的变化进行主动外排机制的初步确证。结果75株鲍曼不动杆菌对临床常用抗生素均有不同程度耐药,CCCP处理前后MIC值均呈现不同程度变化,多种药物泵阳性株和泵阴性株的耐药率和敏感率差异有统计学意义(P<0.05)。结论临床鲍曼不动杆菌耐药形势严峻,75株试验菌株存在药物主动外排机制,其高度耐药性及多重耐药性可能与主动外排机制密切相关。

Objective To analyze the drug resistance of clinical separated Acinetobacter baumannii in our hospital,and to explore whether Acinetobacter baumannii efflux antibacterials actively.Methods K-B method was used to determine the drug resistance of clinical separated Acinetobacter baumannii.Influence of carbonyl cyanidem-chlorophenylhydrazone （CCCP） in Acinetobacter baumannii on minimal inhibitory concentrations （MICs） of various anti-microbial agents was detected to identify active efflux mechanism.Results Seventy-five strains of the clinical separated Acinetobacter baumannii had different-level resistance to antibacterials.CCCP had effect on the MICs of conventional anti-microbial agents to sensitive and resistant anti-microbial agents Acinetobacter baumannii strains.There were statistically significant differences in drug resistance rate and sensitive rate of positive and negative efflux pump strains（P0.05）.Conclusions Situation of drug resistance is serious in the clinical separated Acinetobacter baumannii.Active drug efflux mechanism exists in 75 strains.High-level and multi-drug resistance may be closely associated with active drug efflux mechanism.