宫颈癌人乳头状瘤病毒感染与P53蛋白表达的关系

Relation between Human Papillomavirus Infection and P53 Protein Expression in Cervical Carcinoma

采用聚合酶链反应对４９例宫颈癌组织石腊包埋标本中人乳头状瘤病毒（ＨＰＶ）６、１１、１６、１８型ＤＮＡ进行检测，并与本研究组曾报导的同标本中Ｐ５３蛋白检测结果进行比较分析。结果表明：①ＨＰＶ—ＤＮＡ的检出（８７．７６％）分别与ＨＰＶ６、１１、１８—ＤＮＡ的检出（２６．５２％、３６．７３％、３８．７８％）相比有显著性差异（Ｐ＜０．００１）。②ＨＰＶ各型间有交叉感染，单独ＨＰＶ１６感染与各型间交叉感染相比有显著性差异（Ｐ＜０．０５～０．００１）。③ＨＰＶ各型感染与否与Ｐ５３蛋白阳性与否无显著相关性（Ｐ＞０．７），但ＨＰＶ总感染率与Ｐ５３蛋白阳性率相比有显著性差异（Ｐ＜０．００１），在Ｐ５３蛋白阳性标本中，ＨＰＶ—ＤＮＡ阳性与阴性相比有显著性差异（Ｐ＜０．００１）。这些结果对阐明ＨＰＶ感染后宫颈癌的发病机理及宫颈癌的早期预测。

Human papillomavirus (HPV)6,11,16,18—DNA were examined by polymerase chain reaction(PCR) in the 49 cases of cervical carcinoma paraffin-embedded tissue specimens and were analysed in contrast to the results of P53 protein expression in the same specimens that had been reported.Results showed that the positive rates of HPV16—DNA(87.76%) was higher than that of HPV6,11,18—DNA(26.52%,36.73%,38.78%)(P<0.001),and the overlapping infections of the four HPVs were existed in the cervical carcinoma,only HPV16 infection was higher than other HPV types overlapping infection(P<0.05～0.001),and the positive rates of HPV—DNA was higher than the negative rates of HPV—DNA(P<0.001) in the positive specimens of P53 protein expression. These results have a certain signiticance to explain the pathogenesis of the cervical carcinoma after the HPV infection and early to forecast and diagnosis the cervical carcinoma.