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香烟烟雾提取物对A549细胞β防御素-2表达的影响 被引量：3

Effects of Cigarette Smoke Extract on the Expression of Human β-defensin 2 in A549 Cell
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摘要 目的研究香烟烟雾提取物(cigarette smoke extract,CSE)对人肺腺上皮细胞A549β防御素-2(humanβ-defensin2,hBD-2)表达的影响。方法培养A549细胞,加或不加LPS(10μg/ml)情况下用5%CSE进行干预。用RT-PCR法和Western Blot法分别检测hBD-2mRNA和蛋白表达变化。结果LPS可以诱导A549细胞hBD-2的表达;5%CSE不影响A549细胞hBD-2的表达,但可抑制LPS的诱导作用。结论CSE可以抑制LPS诱导的肺腺上皮细胞hBD-2表达,这可能与CSE减弱呼吸道抵御外界微生物入侵能力,导致呼吸道感染及慢性阻塞性肺疾病有关。 Objective To investigate the effects of cigarette smoke extract （CSE） on the expression of human β-defensin 2 （hBD-2） in lung epithelial cell line A549. Methods A549 cells were stimulated with 5%CSE in the presence or absence of LPS. The mRNA and pro- tein expression of hBD-2 were determined by RT-PCR and Western Blot respectively. Results LPS significantly enhanced the expression of hBD-2 in A549 cells. The 5%CSE had no effect on the expression of hBD-2. However, exposure of A549 cells to CSE markedly inhibited the induction ofhBD-2 by LPS both in mRNA level and protein level. Conclusion CSE can suppress the expression of hBD-2 in lung ep- ithelial cells which is induced by LPS, which is related to this： CSE can weaken the air tube defending the invasion on outside microorganisms, which will cause airway infection and chronic obstructive pulmonary disease.
作者 李明 李岩 陈伟强 罗少红
机构地区 广东药学院基础学院 广州中医药大学基础医学院
出处 《临床医学工程》 2010年第4期4-6,共3页 Clinical Medicine & Engineering
基金 广东药学院师资队伍建设经费 广东省高校重点培养青年教师基金(编号:51343003)
关键词 香烟烟雾提取物 Β防御素-2 肺腺上皮细胞 Cigarette smoke extract Human β-defensin 2 Lung epithelial cells
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献12

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二级参考文献19

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共引文献6

同被引文献23

  • 1张永,程德云,王慧,苏巧俐,陈小菊,关键.慢性阻塞性肺疾病大鼠肺内白细胞介素-8和肿瘤坏死因子-α与气道炎症的关系研究[J].中国呼吸与危重监护杂志,2003,2(6):355-359. 被引量：41
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临床医学工程
2010年 第4期

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