摘要
目的建立HPLC-MS／MS法同时测定人血浆中的安非他酮及其代谢产物4-OH-安非他酮的浓度。方法血浆样品经乙腈沉淀蛋白后，采用Hypurity C18柱（150mm×2．1mm，5μm）以乙腈-0．1％甲酸-10mm01．L。甲酸铵缓冲液（65：10：25）为流动相，流速为0．2mL·min，通过电喷雾离子化离子阱质谱，以二级质谱选择离子反应监测（SRM）方式进行检测。结果安非他酮线性范围为1．172～1200ng·mL^-1，4-OH-安非他酮线性范围为0．42-430ng·mL^-1,安非他酮和4-OH-安非他酮最低检测浓度分别低于1．0ng·mL^-1和0．21ng·mL^-1结论本法特异性强、灵敏度高，操作简便快速，测定结果可靠，可以准确地测定人体血浆安非他酮和4-OH-安非他酮的浓度。
Objective To establish an HPLC-MS/MS method for the simultaneous determination of amfebutamone and 4-OH-amfebutamone in human plasma. Methods After precipitation with acetonitrile, plasma sample was separated on a Hypurity C18 column （ 150 mm×2.1 mm, 5μm ）, with acetonitrile-0.1% formate acid -10 mmol ·mL^-1 ammonium formate （65 ： 10 ： 25） as the mobile phase, the flow rate was 0. 2 mL·min^-1. The eletronspray ion trap mass spectrum in the positive mode and selected reaction monitor （ SRM ） were used to detect the concentration of roxithromycin. Results The linear range of amfebutarnone and 4-OH- amfebutamone was 1. 172-1 200 ng·mL^-1 and 0. 42--430 ng·mL^-1. The limitation of detection for amfebutamone and 4 OH amfebutamone were 1.0 ng·mL^-1 and 0.21 ng·mL^-1, respectively. Conclusion This method is accurate, sensitive and simple, and suitable for the determination of amfebutamone and 4-OH amfebutamone.
出处
《中南药学》
CAS
2010年第4期282-285,共4页
Central South Pharmacy
