摘要
目的建立骨伤愈合胶囊的定性定量方法。方法采用薄层色谱法对处方中乳香、没药、当归、大黄进行定性鉴别,采用高效液相色谱法测定阿魏酸的含量。色谱柱为phenomenex ODS C18(4.6mm×200mm,5μm)柱,流动相为甲醇~1%磷酸溶液(30:70);检测波长320nm;流速为1.0mL·min^-1,柱温为25℃。结果薄层色谱鉴别方法专属性强,阿魏酸在浓度4.85-48.5μg·mL^-1与峰面积呈良好的线性关系(r=0.9999),平均回收率为96.9%,RSD=2.21%(n=9)。结论本方法准确、简便、专属性好,可用于骨伤愈合胶囊的定性定量质量控制。
Objective To establish a qualitative and quantitative method for Gushangyuhe capsules. Methods Boswellin, myrrh, Radix Angelicae Sinensis and Rhubarb in recipes were identified by TLC. The content of ferulaic acid was determined by HPLC. The chromatographic column was phenomenex ODS C18 (200 min× 4.6 mm, 5μm). The mobile phase consisted of methanol-1% phosphoric acidwater solution (30 : 70), and the flow rate was 1.0 mL · min^-1. The detection wavelength was 320 nm and the column temperature was 25℃. Results The TLC identification was highly specific; the amount of ferulaic acid had a good linearity over 4. 85--48.5μg·mL^-1. The average recovery of syringin was 96.9% and RSD was 2.21G (n=9). Conclusion The method is reliable, simple, accurate and reproducible, and can be used for the quality Control of Gushangyuhe capsules.
出处
《中南药学》
CAS
2010年第4期289-292,共4页
Central South Pharmacy