铜绿假单胞菌注射液对人乳腺癌细胞的体外杀伤及促凋亡作用研究

Effect of pseudomonas aeruginosa vaccine on cell apoptosis and proliferation in human breast cancer cell line MCF-7

目的研究铜绿假单胞菌(pseudo-monas aeruginosa,PA-MSHA)注射液在体外实验中对人乳腺癌细胞(MCF-7)凋亡和增殖的影响,并探讨其可能的作用机制。方法应用MTT比色法检测铜绿假单胞菌注射液对体外培养的MCF-7细胞增殖抑制作用,应用流式细胞术检测铜绿假单胞菌注射液诱导乳腺癌细胞凋亡,检测其对细胞周期的影响。用Hoechst 33258染色法染色,荧光显微镜检测铜绿假单胞菌注射液诱导体外培养的MCF-7肿瘤细胞凋亡的形态学改变。结果铜绿假单胞菌注射液抑制MCF-7细胞增殖,呈剂量和时间依赖性;与对照组相比,铜绿假单胞菌注射液组MCF-7细胞的凋亡率明显增高(P<0.01)、G0/G1期细胞比例明显增高、S期细胞所占百分比明显降低(P<0.05),染色荧光显微镜检测发现PA-MSHA能诱导体外培养的肿瘤细胞呈现明显的凋亡图像。结论铜绿假单胞菌注射液可能通过对细胞周期中G0/G1期阻滞,诱导MCF-7细胞凋亡,从而抑制MCF-7细胞的增殖。

Objective To investigate the effect of PA-MSHA vaccine on the apoptosis and proliferation of human breast cancer cells MCF-7 cultured in vitro and to discuss the possible mechanism. Methods After MCF-7 was treated with different concentrations of PA-MSHA vaccine for different time, MTT colorimetric assay was applied to examine the growth inhibition and the growing curves were drawra Then, the apoptotsis rate and the change of cell cycle of breast cancer cells were detected by flow cytometry. The apoptotic features was observed with fluorescence microscope after stained by Hoechst 33258. Results PA-MSHA vaccine could inhibit the proliferation of MCF-7 cells in a time and dose-dependent manner. The flow cytometric analysis of the cell cycle distribution revealed that the tumor cell apoptotsis rate in the PA-MSHA vaccine group was significantly higher than that in the control group （P〈0.01） , and the fraction of S phase cells decreased, while the fraction of Go/G1 phase cells increased compared with that in the control group （P〈 0. 05）. The significant apoptotic picture was obtained with fluorescence microscope. Conclusion PAMSHA vaccine can cause tumor cell apoptosis, inhibit the growth proliferation in MCF7 cells, and arrest them at G1 phase. Apoptosis induction may be the important mechanism for anti-tumor action of PAMSHA vaccine.