冷藏胚胎骨的组织结构及BMP的免疫组化观察

Histological and BMP Immunohistochemical Observation on Cryopreserved Embryo Bone

用免疫组织化学及组织学方法，观察了－２０℃冷藏６～８个月的人胚胎长骨的组织结构与骨形态发生蛋白（ＢＭＰ）的保存情况，并与同体胚胎新鲜骨进行了对比。发现－２０℃冷藏６～８个月的胚胎骨中，骨髓细胞大部分失活、死亡，骨细胞部分死亡、消失，但破骨细胞、成骨细胞保存较好，ＢＭＰ保存良好。认为用－２０℃冷藏半年以上的胚胎骨作骨移植，既可避免活的免疫原性细胞所激发的强烈的免疫反应。

The remains of BMP in human embryo long bone cryopreserved for 6～8 months at -20℃ and its construction were observed with immunohistochemical and histological methods. The results were comparaed with those of fresh bone of the same embryo. It was found that the greater part of bone marrow cells were vigourless or died out, part of bone cells died and disappeared, but the osteoblasts and osteoclasts were conserved better, the BMP was preserved good in this refrigerated embryo bone. the authors suggest that transplantation with embryo bone cryopreserved for more than six months at the temperature of -20℃ might avoid intense immunologic rejection evoked by the live immunogenicity cells as well as perserve its ability of bone induction better.