甲氨喋呤抑制寻常型银屑病患者外周血MMP-9及VEGF的表达

Effects of methotrexate on serum expression of MMP-9 and VEGF in patients with psoriasis vulgaris

目的探讨甲氨喋呤(Methotrexat,MTX)对寻常型银屑病患者外周血中基质金属蛋白酶9(Matrix metalloproteinase 9,MMP-9)及血管内皮生长因子(Vascular endothelial growth factor,VEGF)表达的调控作用。方法采用酶联免疫吸附试验(ELISA),检测22例寻常型银屑病患者外周血治疗前、MTX治疗4周后及20例正常对照者外周血MMP-9及VEGF的表达。结果MMP-9及VEGF在寻常型银屑病外周血中治疗前的表达水平分别明显高于健康对照者的表达(t=6.59,P<0.001;t=8.98,P<0.001);患者经过MTX治疗4周后,MMP-9及VEGF的表达明显下降(t=5.79,P<0.001;t=7.06,P<0.001),与正常对照者的表达水平差异无统计学意义(t=1.24,P=0.2237;t=1.38,P=0.1755)。结论MTX抑制寻常型银屑病外周血MMP-9及VEGF的表达,可能是MTX治疗银屑病的作用机制之一。

Objective To investigate the effects of methotrexate on serum expression of matrix metalloproteinase 9（MMP-9）and vascular endothelial growth factor（VEGF）in patients with psoriasis vulgaris.Methods Twenty-two adult patients with psoriasis vulgaris and 20 health control were sequentially enrolled in this study.Serum levels of MMP-9 and VEGF were measured by enzyme linked immunosorbent assay（ELISA）in 22 cases with psoriasis vulgaris before and after treatment with MTX for 4 weeks and 20 health control.Results The serum levels of MMP-9 and VEGF before treatment were significantly higher than that after treatment and health control respectively（t=6.59,P0.001;t=8.98,P0.001）.Expression of serum MMP-9 and VEGF in psoriasis decreased after 4 weeks methotrexate treatment respectively（t=5.79,P0.001;t=7.06,P0.001）,and there was no significant difference in serum levels of MMP-9 and VEGF,compared with health control group（t=1.24,P=0.223 7[t=1.38,P=0.175 5）.Conclusion MTX can inhibit the over expression of MMP-9 and VEGF in patients with psoriasis vulgaris.