补肾活血方对大鼠佐剂性关节炎疗效的实验研究

Experimental Study of Bushen Huoxue Formula on the Efficacy of Adjuvant Arthritis in Rats

目的观察补肾活血方对大鼠佐剂性关节炎的治疗作用,并探讨其作用机制。方法将48只大鼠,按体重随机分为补肾活血方高剂量组(高剂量组)、补肾活血方中剂量组(中剂量组)、补肾活血方低剂量组(低剂量组),雷公藤组,模型组,正常对照组,每组8只。除正常对照组外,各组用完全弗氏佐剂造模,造模后各治疗组给补肾活血方水溶液灌胃,雷公藤组用雷公藤多苷片水溶液灌胃,正常对照组用蒸馏水灌胃。观测关节炎指数(AI)、足跖肿胀厚度、体重(W)的改变,测定血清免疫球蛋白(IgG、IgM)、肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)的含量。结果与模型组比较,补肾活血方各治疗组抑制左、右足跖肿胀和AI升高,TNF-α、IgG、IgM、IL-1的含量明显下降(P<0.01,P<0.05)。结论补肾活血方有治疗大鼠佐剂性、继发性关节炎的作用,其机制可能与抑制自身免疫的病理性反应有关。

Objective To observe the therapeutic action of bushen huoxue formula（the formula for tonifying kidney and activating blood circulation,hereinafter referred to＂formula＂）on adjuvant arthritis in rats and to explore its mechanism.Methods 48 rats were randomly divided into high-dose formula group,middle-dose group,low-dose group,triptolide treatment group,model group and normal control group,8 rats in each one.Except the normal control group,Freund＇s adjuvant complete modeling method was applied in the other groups.After modeling,the formula decoction was applied for gastric lavage in treatment groups,TWP-chip solution was used for gastric lavage in triptolide treatment group and the distilled water was used in normal control group.The arthritis index（AI）,the thickness of paw edema,and weight（W）changes were observed.The serum immunoglobulin（IgG,IgM）,tumor necrosis factor-α（TNF-α）,interleukin-1（IL-1）contents were measured.Results Compared with the model group,in each treatment group with the formula administered,the plantar swelling of feet and AI increasing were inhibited,and the levels of TNF-α,IgG,IgM and IL-1 decreased significantly（P0.01 or P0.05）.Conclusion Bushen huoxue formula acts on adjuvant and secondary arthritis in rats and its mechanism may be related to the suppression of pathological autoimmune responses.